https://scholars.lib.ntu.edu.tw/handle/123456789/175980
標題: | 組織培養苗生產系統環境控制(二) Environmental control on the production of tissue culture plantlets in vitro |
作者: | 方煒 | 關鍵字: | 二氧化碳施肥;金線連;自營生 長;即時監控;組織培養;CO2 enrichment;Anoectochilus formosanus Hayata;photoautotrophic growth;real time monitoring and control;tissue culture | 公開日期: | 31-七月-1998 | 出版社: | 臺北市:國立臺灣大學生物產業機電工程學系暨研究所 | 摘要: | 本研究旨在建立一適用於組織培 養瓶苗栽培的CO2 補充,溫、濕度與 CO2 濃度即時監測與控制的系統。探 討二氧化碳施肥對本土的珍貴藥材 - 台灣金線連(Anoectochilus formosanus Hayata)組培苗自營生長 (photoautotrophic growth) 之影響為 本研究的第二目的。試驗結果顯示監 控系統之運作合乎需求, 並且在60 天的栽培中,對照組(傳統栽培方式) 的相對生長速率為1.78%/日,控制組 (CO2 施肥組)則為3.73%/日,增加倍 數達2.1 倍。證實在光期對透氣性良 好且培養基中不加糖的金線連組培 瓶補充二氧化碳確可達到提高瓶內 組培苗生長速率之目的,且台灣金線 連組培苗採自營方式栽培亦證實為 可行。 The first purpose of this study was to develop a real time CO2 supplying, temperature, relative humidity and CO2 concentration monitoring and control system for the growth of tissue culture plantlets in vitro. The second purpose was to examine the effects of CO2 enrichment on the photoautotrophic growth of Anoectochilus formosanus Hayata , a traditional Taiwanese galenical plants, plantlets in vitro. The results show that after the 60 days of growth, the relative growth rate of the group using the traditional method is 1.78% per day and is 3.73% per day for the CO2 enriched group. The growth rate was improved by 2.1 times. The results of the experiment proved that the CO2 enrichment is useful in increasing the growth rate of Anoectochilus formosanus Hayata plantlets in vitro and to culture the plantlets of interests in vitro photoautotrophically is possible. The monitoring and control system developed in this study worked well and functioned properly throughout the entire experiment. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/10410 | 其他識別: | 872313B002070 | Rights: | 國立臺灣大學生物產業機電工程學系暨研究所 |
顯示於: | 生物機電工程學系 |
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872313B002070.pdf | 66.8 kB | Adobe PDF | 檢視/開啟 |
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