醣苷水解酶活性探針化學合成之探討

Abstract

醣苷水解酶在生物體中扮演重要的角色。此類的酶可以催化醣苷鍵的形成或斷裂，而這一類的鍵結通常會在澱粉、植物細胞的細胞壁中出現。醣苷水解酶的種類多且複雜，已經被分類出好幾個家族，近來也有研究指出，當某一類的醣苷水解酶表現異常時，會有某種病變發生，已知的有Fabry’s disease、HIV disease…等。針對醣苷水解酶所設計的活性探針，將會提供一個有力的生物工具來協助研究醣苷水解酶在生物體內的分布及活性。 合成方面，由於辨識端的醣類可分為ㄐBβ兩種類型，三氟化硼及銀鹽的使用使得控制辨識端的立體位向變的容易。所以在針對不同的目標醣苷水解酶如ㄚ爰眶敹}水解酶…等等可以合成出不同的活性探針。 活性探針的設計主要是分成四大部分(1)辨識端(2)捕捉端(3)連接橋端(4)發報端，每個部分都各司其職。由於研究的對象是醣苷水解酶，故選擇了醣類來當作辨識端。

Glycosidases play an important role in living organism. They can catalyze the formming or breaking glycosidic bond, and this kind of bonding is usually in the form of starch or component of cell wall of plants. According to the recent researches, there are some sorts of diseases caused from abnormal expression of glycosidases, such as Fabry’s disease and HIV disease. Therefore, activity probes for labeling glycosidases will be a powerful tool in this field. Especially studying the activity and distribution in living organism. Because of