Immobilization of Biomimic complex ofuperoxide Dismutase in Mesoporous Material
Date Issued
2008
Date
2008
Author(s)
Lin, Han-Chou
Abstract
Superoxide dismutases (SODs) are found in prokaryotes,itochondria iron and plants, and the copper -zinc SODs are most frequent in eukaryotic cells. Cells are normally able to prevent DNA damage and apoptosis by defending themselves against reactive oxygen species(ROS) damage through the use of enzymes such as superoxide dismutases and catalases. We have synthesized a biomimic complex of CuZnSOD by imitating of its structure of active site. The spectrum of UV and IR could provide the characteristics of this mimic complex, and activity assay counld be conducted via an indirect competition method. The IC 50 of the mimic complex is about 55μM. Immobilizing the mimic complex into NaY and mesoporous materials through electronic and covalent bond would result in different activity and stability in vitro. We found that materials modified with electronic bond would generally raise its activity due to their larger freedom than those modified with covalent bond. In vivo, MSN -FITC had been proved its cell permeability. Modifying MSN -FITC with mimic complex could enter cells to eliminate superoxide and to lower the live function index of male mice stressed by acetaminophen.
Subjects
Superoxide dismutase
Mesoporous
Biomimic
Type
thesis
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