Skip navigation
  • 中文
  • English

DSpace CRIS

  • DSpace logo
  • Home
  • Organizations
  • Researchers
  • Research Outputs
  • Explore by
    • Organizations
    • Researchers
    • Research Outputs
  • Academic & Publications
  • Sign in
  • 中文
  • English
  1. NTU Scholars
  2. 理學院
  3. 化學系
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/178523
Title: 中孔洞二氧化矽材料附載生物模擬超氧歧化酵素
Immobilization of Biomimic complex ofuperoxide Dismutase in Mesoporous Material
Authors: 林漢洲
Lin, Han-Chou
牟中原指導 
Keywords: 超氧歧化酵素;中孔洞;生物模擬;Superoxide dismutase;Mesoporous;Biomimic
Issue Date: 2008
Abstract: 
超氧歧化酵素(superoxide dismutase)普遍存在人體和動、植物細胞。一般生理上會引起ROS(Reactive Oxygen Species) Stress,進而傷害細胞、破壞DNA,甚至造成細胞凋亡(Apoptosis),在此超氧歧化酵素扮演著很重要的清除超氧離子的抗氧化作用。經由模擬超氧歧化酵素的活性中心結構,合成了一個與它相近的生物模擬CuZn 金屬錯合物(Biomimic complex),可以從UV, IR Spectrum 觀察出它的結構特徵,另外也由一種間接的競爭方式測出它的活性表現IC50 約為55 μM。In vitro 方面,藉由將這個生物模擬CuZn 金屬錯合物(Biomimic complex)附載到各種不同的中孔洞二氧化矽材料(Al-MCM41,48, MAS9, MSN…等),研究其附載上去之後的活性、穩定性表現,進一步再比較使用共價鍵和離子鍵等不同的修飾方法,在活性上的差異性,發現部份離子鍵的修飾法活性相對較高,甚至達到約3 倍的提昇效果。n vivo方面,藉由本實驗室之前所開發出來的修飾有FITC螢光物中孔洞奈米粒子(MSN-FITC),之前已確定它具有進入細胞的能力,是將生物模擬CuZn金屬錯合物(Biomimic complex)修飾上去,進一步去研究它們在細胞中的毒性測試,活性表現、降低老鼠肝指數等方向。

Superoxide dismutases (SODs) are found in prokaryotes,itochondria iron and plants, and the copper -zinc SODs are most frequent in eukaryotic cells. Cells are normally able to prevent DNA damage and apoptosis by defending themselves against reactive oxygen species(ROS) damage through the use of enzymes such as superoxide dismutases and catalases. We have synthesized a biomimic complex of CuZnSOD by imitating of its structure of active site. The spectrum of UV and IR could provide the characteristics of this mimic complex, and activity assay counld be conducted via an indirect competition method. The IC 50 of the mimic complex is about 55μM. Immobilizing the mimic complex into NaY and mesoporous materials through electronic and covalent bond would result in different activity and stability in vitro. We found that materials modified with electronic bond would generally raise its activity due to their larger freedom than those modified with covalent bond. In vivo, MSN -FITC had been proved its cell permeability. Modifying MSN -FITC with mimic complex could enter cells to eliminate superoxide and to lower the live function index of male mice stressed by acetaminophen.
URI: http://ntur.lib.ntu.edu.tw//handle/246246/187414
Appears in Collections:化學系

Files in This Item:
File Description SizeFormat
ntu-97-R94223010-1.pdf23.32 kBAdobe PDFView/Open
Show full item record

Page view(s)

25
checked on Dec 17, 2020

Download(s) 10

2
checked on Dec 17, 2020

Google ScholarTM

Check

Related Items in TAIR


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Sherpa Romeo網站查詢,以確認出版單位之版權政策。
    Please use Sherpa Romeo to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)
Build with DSpace-CRIS - Extension maintained and optimized by Logo 4SCIENCE Feedback