System Peaks of Cyclodextrins in Capillary Electrophoresis
Resource
Journal of High Resolution Chromatography 23 (6): 409-412
Journal
Journal of High Resolution Chromatography
Pages
409-412
Date Issued
2000
Date
2000
Author(s)
Chen, Yet Ran
Ju, Dar Der
Her, Guor Rong
Abstract
Using a running buffer containing cyclodextrins (CDs) and 2-[N-cyclohexylamino]-ethanesulfonic acid (CHES), positive system peaks were observed in the analysis of a ganglioside mixture by CE-UV. These system peaks were related to CDs in the running buffer because these peaks were also detected when a plug of solution devoid of CDs but having the same CHES concentration and pH as the running buffer was injected. Neutral CDs were separated owing to the formation of inclusion complexes with the anionic CHES ion. One possible explanation for the positive system peaks is that the anionic CD-CHES inclusion complex is displaced by co-ions with higher UV absorptivity.
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
32.pdf
Size
43.93 KB
Format
Adobe PDF
Checksum
(MD5):01efcd323f95779f3008aec2bdeb77d4