https://scholars.lib.ntu.edu.tw/handle/123456789/181436
標題: | Nanoprobe-Based Affinity Mass Spectrometry for Selected Protein Profiling in Human Plasma | 作者: | Chou, Po-Hung Chen, Shu-Hua Liao, Hsin-Kai Lin, Po-Chiao Her, Gour-Rong Lai, Alan Chuan-Ying Chen, Jenn-Han Lin, Chun-Cheng Chen, Yu-Ju |
公開日期: | 2005 | 起(迄)頁: | 5990-5997 | 來源出版物: | Analytical Chemistry | 摘要: | In recent decades, magnetic nanoparticles have emerged as a promising new platform in biomedical applications, particularly bioseparations. We have developed an immunoassay using antibody-conjugated magnetic nanoparticles as an efficient affinity probe to simultaneously preconcentrate and isolate targeted antigens from biological media. We combined this probe with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI MS) to profile proteins in diluted human plasma. The nanoparticles were designed to detect several disease-associated proteins and could be used directly in MALDI MS without an elution step, thereby facilitating multiple antigen screening and the characterization of antigen variants. Plasma antigens bound rapidly (?10 min) to the antibody-conjugated nanoparticles, allowing the assay to be performed within 20 min. With sensitivity of detection in the femtomole range, the nanoscale immunoassay is superior to assays using microscale particles. We applied our method to comparative protein profiling of patients with gastric cancer and healthy individuals and found differential protein expression levels associated with the disease as well as individuals. Given the flexibility of manipulating functional groups on the nanoprobes, their low cost, robustness, and simplicity of the assay, our approach shows promise for targeted proteome profiling in clinical settings. ? 2005 American Chemical Society. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/166286 | DOI: | 10.1021/ac050655o | SDG/關鍵字: | Gastric cancer; Human plasma; Magnetic nanoparticles; Protein profiling; Antibodies; Antigens; Bioassay; Biomedical engineering; Diseases; Immunization; Nanostructured materials; Proteins; Mass spectrometry; C reactive protein; cytochrome c; enolase; myoglobin; nanoparticle; plasma protein; serum amyloid P; sinapic acid; analytic method; antibody affinity; article; blood sampling; case report; controlled study; cross linking; enzyme linked immunosorbent assay; human; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; particle size; plasma; protein blood level; protein expression; proteomics; stomach cancer; Antibodies; Antigens; Blood Proteins; Humans; Kinetics; Mass Spectrometry; Microscopy, Electron, Transmission; Nanoparticles; Sensitivity and Specificity |
顯示於: | 化學系 |
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