https://scholars.lib.ntu.edu.tw/handle/123456789/184078
標題: | Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles | 作者: | Chiang, Cheng-Kang Lin, Yang-Wei Chen, Wen-Tsen Chang, Huan-Tsung |
關鍵字: | Aminothiols; Drug therapy; Gold nanoparticles; Internal standard; Mass spectrometry | 公開日期: | 2010 | 卷: | 6 | 期: | 4 | 起(迄)頁: | 530-537 | 來源出版物: | Nanomedicine Nanotechnology Biology and Medicine | 摘要: | We developed a method for the determination of three aminothiols-cysteine, glutathione (GSH), and homocysteine-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine-modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ~0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes. From the Clinical Editor: This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys). ? 2010 Elsevier Inc. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/239344 http://ntur.lib.ntu.edu.tw/bitstream/246246/239344/-1/330.pdf |
DOI: | 10.1016/j.nano.2010.01.006 | SDG/關鍵字: | Aminothiols; Analytes; Anti-inflammatory drugs; Glutathiones; Gold Nanoparticles; Homocysteines; Human red blood cell; In-cell; Internal standards; Lysates; Quantitative determinations; Relative standard deviations; Reproducibilities; Surface-assisted laser desorption/ionization; Blood; Desorption; Gold; Mass spectrometry; Nanoparticles; Standards; Drug therapy; cysteine; glutathione; gold nanoparticle; homocysteine; salazosulfapyridine; tiopronin; accuracy; article; cell lysate; cell strain MCF 7; controlled study; erythrocyte; human; human cell; mass spectrometry; particle size; quantitative analysis; reproducibility; standard; surface assisted laser desorption ionization mass spectrometry; Erythrocytes; Glutathione; Gold; Homocysteine; Humans; Metal Nanoparticles; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tumor Cells, Cultured |
顯示於: | 化學系 |
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