https://scholars.lib.ntu.edu.tw/handle/123456789/184097
標題: | Analgesic Effects and the Mechanisms of Anti-inflammation of Ergostatrien-3 beta-ol from Antrodia camphorata Submerged Whole Broth in Mice | 作者: | Huang, Guan-Jhong Huang, Shyh-Shyun Lin, Shiang-Shiou Shao, Yi-Yuan Chen, Chin-Chu Hou, Wen-Chi Kuo, Yueh-Hsiung |
關鍵字: | Analgesic; Anti-inflammation; Chinese herb; Ergostatrien-3β-ol; MDA; NO; TNF-α | 公開日期: | 2010 | 卷: | 58 | 期: | 12 | 起(迄)頁: | 7445-7452 | 來源出版物: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 摘要: | Ergostatrien-3β-ol (ST1), an active and major ingredient from Antrodia camphorata (AC) submerged whole broth was evaluated for the analgesic and anti-inflammatory effects. Treatment of male imprinting control region (ICR) mice with ST1 (1, 5, and 10 mg/kg) significantly inhibited the numbers of acetic-acid-induced writhing response in 10 min. Also, our result showed that ST1 (10 mg/kg) significantly inhibited the formalin-induced pain in the late phase (p < 0.001). In the anti-inflammatory test, ST1 (10 mg/kg) decreased the paw edema at 4 and 5 h after λ-carrageenin (Carr) administration and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the liver tissue. We also demonstrated that ST1 significantly attenuated the malondialdehyde (MDA) level in the edema paw at 5 h after Carr injection. ST1 (1, 5, and 10 mg/kg) decreased the nitric oxide (NO) levels on both the edema paw and serum level at 5 h after Carr injection. Also, ST1 (5 and 10 mg/kg) diminished the serum tumor necrosis factor (TNF-α) at 5 h after Carr injection. Western blotting revealed that ST1 (10 mg/kg) decreased Carr-induced inducible nitric oxide synthase (iNOS), and cycloxyclase (COX-2) expressions at 5 h in the edema paw. An intraperitoneal (ip) injection treatment with ST1 also diminished neutrophil infiltration into sites of inflammation, as did indomethacin (Indo). The anti-inflammatory mechanisms of ST1 might be related to the decrease in the level of MDA, iNOS, and COX-2 in the edema paw via increasing the activities of CAT, SOD, and GPx in the liver through the suppression of TNF-α and NO. ? 2010 American Chemical Society. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/239363 | DOI: | 10.1021/jf1013764 | SDG/關鍵字: | analgesic agent; antiinflammatory agent; carrageenan; cyclooxygenase 2; inducible nitric oxide synthase; malonaldehyde; analgesic agent; antiinflammatory agent; animal; Antrodia; article; chemically induced disorder; chemistry; disease model; drug effect; edema; gene expression; genetics; human; immunology; Institute for Cancer Research mouse; male; metabolism; mouse; pain; randomization; Antrodia; chemically induced; chemistry; drug effects; edema; pain; Analgesics; Animals; Anti-Inflammatory Agents; Antrodia; Carrageenan; Cyclooxygenase 2; Disease Models, Animal; Edema; Gene Expression; Humans; Male; Malondialdehyde; Mice; Mice, Inbred ICR; Nitric Oxide Synthase Type II; Pain; Random Allocation; Mus; Taiwanofungus camphoratus; Analgesics; Animals; Anti-Inflammatory Agents; Antrodia; Carrageenan; Cyclooxygenase 2; Disease Models, Animal; Edema; Gene Expression; Humans; Male; Malondialdehyde; Mice; Mice, Inbred ICR; Nitric Oxide Synthase Type II; Pain; Random Allocation |
顯示於: | 化學系 |
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