整合型總計畫名稱：溫度逆境下植物基因產物 蛋白質的分子研究 本計畫名稱：水稻低溫耐性基因構造與功能之研究
The gene structure and function of rice in chilling tolerance
Date Issued
2000-07-31
Date
2000-07-31
Author(s)
DOI
892313B002113
Abstract
Rice (Oryza sativa L.) seedlings
frequently suffer from chilling injury during
early spring in Taiwan as well as in other
subtropical areas. The chilling injury may
cause severe growth retardation and yield
reduction. To gain more insights of molecular
mechanisms of chilling tolerance, cDNAs
associated with chilling tolerance in rice
seedlings were isolated using differential
display of mRNA. Seedlings of a chillingsensitive
indica type cultivar (cv. Taichung
Native 1, TN 1), and a chilling-tolerant
japonica type cultivar (cv. Tainung 67, TNG
67) were treated for 24 h with either: (1) 5oC;
(2) 25oC (control); (3) 10 mM ABA at 25oC;
or (4) 10 mM methyl jasmonate (MeJa) at
25oC. The later two treatments were
previously found that could induce chillingresistance
in TN1 seedlings. Total RNA were
isolated from the roots of seedlings and used
for differential display. Clones of 362 cDNA
were re-amplified and isolated from 600
differentially expressed cDNA fragments.
Sequences of 153 cDNA clones had
significant similarities to the sequences
known in the GenBank. Sixty-one of these
clones, which could be separated to 29
groups, showed high homology to the
sequences with known functions, i.e.,
rab16D, heat shock protein, SalT, and Zninduced
protein.
Slot-blot analysis revealed that 58 cDNA clones could be regulated by ABA, MeJA or
chilling treatments. Among these 58
differentially expressed cDNA clones, 28
cDNA clones could be enhanced by ABA or
chilling using northern blot analysis. In
addition, 6 clones of the 28 clones showed
differential expressions under stresses
including chilling, osmotic, salt, heat shock,
and ABA or MeJa treatments. By testing
their expressions in 11 cultivars, which have
different chilling tolerance and different
chilling-induced ABA levels, six cDNA
clones showed positively correlated with
chilling tolerance. Some of these cDNA
clones showed much higher transcript levels
after exposure to chilling stress in TN 1
seedlings that pre-treated with ABA.
Therefore, these clones might be involved in
the ABA-mediated acclimation of chilling
resistance in TN 1 seedlings. In contrast,
transcript levels of other cDNA clones were
up-regulated in seedlings without ABA pretreatment,
and their expressions seemed not
to be related to endogenous ABA levels.
Subjects
plant hormone
differential display
gene expression
rice
chilling
tolerance
tolerance
abscisic acid
植物荷爾蒙
差異表現法
水稻
低溫耐性
離層酸
基因表現
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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