Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Bioresources and Agriculture / 生物資源暨農學院
  3. Agronomy / 農藝學系
  4. 整合型總計畫名稱:溫度逆境下植物基因產物 蛋白質的分子研究 本計畫名稱:水稻低溫耐性基因構造與功能之研究
 
  • Details

整合型總計畫名稱:溫度逆境下植物基因產物 蛋白質的分子研究 本計畫名稱:水稻低溫耐性基因構造與功能之研究

The gene structure and function of rice in chilling tolerance

Date Issued
2000-07-31
Date
2000-07-31
Author(s)
朱鈞
盧虎生  
陳勝利
DOI
892313B002113
URI
http://ntur.lib.ntu.edu.tw//handle/246246/19818
Abstract
Rice (Oryza sativa L.) seedlings frequently suffer from chilling injury during early spring in Taiwan as well as in other subtropical areas. The chilling injury may cause severe growth retardation and yield reduction. To gain more insights of molecular mechanisms of chilling tolerance, cDNAs associated with chilling tolerance in rice seedlings were isolated using differential display of mRNA. Seedlings of a chillingsensitive indica type cultivar (cv. Taichung Native 1, TN 1), and a chilling-tolerant japonica type cultivar (cv. Tainung 67, TNG 67) were treated for 24 h with either: (1) 5oC; (2) 25oC (control); (3) 10 mM ABA at 25oC; or (4) 10 mM methyl jasmonate (MeJa) at 25oC. The later two treatments were previously found that could induce chillingresistance in TN1 seedlings. Total RNA were isolated from the roots of seedlings and used for differential display. Clones of 362 cDNA were re-amplified and isolated from 600 differentially expressed cDNA fragments. Sequences of 153 cDNA clones had significant similarities to the sequences known in the GenBank. Sixty-one of these clones, which could be separated to 29 groups, showed high homology to the sequences with known functions, i.e., rab16D, heat shock protein, SalT, and Zninduced protein. Slot-blot analysis revealed that 58 cDNA clones could be regulated by ABA, MeJA or chilling treatments. Among these 58 differentially expressed cDNA clones, 28 cDNA clones could be enhanced by ABA or chilling using northern blot analysis. In addition, 6 clones of the 28 clones showed differential expressions under stresses including chilling, osmotic, salt, heat shock, and ABA or MeJa treatments. By testing their expressions in 11 cultivars, which have different chilling tolerance and different chilling-induced ABA levels, six cDNA clones showed positively correlated with chilling tolerance. Some of these cDNA clones showed much higher transcript levels after exposure to chilling stress in TN 1 seedlings that pre-treated with ABA. Therefore, these clones might be involved in the ABA-mediated acclimation of chilling resistance in TN 1 seedlings. In contrast, transcript levels of other cDNA clones were up-regulated in seedlings without ABA pretreatment, and their expressions seemed not to be related to endogenous ABA levels.
Subjects
plant hormone
differential display
gene expression
rice
chilling
tolerance
abscisic acid
植物荷爾蒙
差異表現法
水稻
低溫耐性
離層酸
基因表現
Publisher
臺北市:國立臺灣大學農藝學系暨研究所
Type
report
File(s)
Loading...
Thumbnail Image
Name

892313B002113.pdf

Size

587.4 KB

Format

Adobe PDF

Checksum

(MD5):557f2df4b90624beb0c909d5c1a483df

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science