https://scholars.lib.ntu.edu.tw/handle/123456789/185077
標題: | The Study of Grain Development in Rice: Relationship
between the Expression and Function of Grain Proteins and
Culture Temperature. 水稻榖粒發育之研究: 榖粒蛋白質表現、功能與栽培溫度之關係 |
作者: | 盧虎生 | 關鍵字: | rice;storage protein;temperature;rice grain quality;水稻;貯藏性蛋白質;溫度;稻 米品質 | 公開日期: | 31-七月-2002 | 出版社: | 臺北市:國立臺灣大學農藝學系暨研究所 | 摘要: | 蛋白質表現為稻米榖粒發育與品質的重要 指標。本計畫主要以蛋白質體學的方法, 研究溫度對榖粒蛋白質表現之影響,以及 與米質相關的原因。本期試驗以日本型水 稻品種(台農67 號,TNG 67)與印度型品種 (台中在來1 號,TN 1)進行研究。將兩品種 分別種植於盆缽並生長於日夜溫30/25℃ 環境,開花後分別處理日夜溫35/30℃, 30/25℃, 20/15℃環境,於開花後6,9,12,15 天取樣並進行分析。利用高解析力的蛋白 質雙向電泳以及LC/MS/MS 胺基酸序列分 析方法,分析在不同溫度環境與稻米品質 下,具有差異表現的蛋白質分子。 利用蛋白質表現差異及蛋白質體分析後, 在現有的水稻蛋白質資料庫或EST 資料庫 中,完成了約30 個蛋白質的序列分析與定 義。其中包括pyruvate orthophosphate dikinase, ER luminal binding protein (BiP), protein disufide isomerase, Waxy proteins, chaperone, enolase 等。此外,有一個分子量 約為28 kDa 的蛋白質可能與稻米心腹白的 形成有關。另一方面,我們發現水稻Waxy 蛋白質具有8-10 isoforms,且其中四個 isoforms 的表現量與稻米榖粒直鏈澱粉含 量呈正相關。結果也顯示台農67 號對高溫 較台中在來1 號敏感。我們期望能進一步 定義出在榖粒發育過程中具有關鍵表現的 蛋白質。 Proteins expressions are important to the development and quality of rice grains. The objectives of this study have been to establish a proteomic study system, and to investigate the temperature effects on the expressions of grain proteins and its relationship with grain quality. A japonica cultivar (Tainung 67, TNG 67) and an indica cultivar (Taichung Native 1, TN 1) were grown in pots at 30/25 ℃ (day/night). At flowering the plants were moved to 35/30℃, 30/25℃, and 20/15℃ for temperature treatments. Grains were then sampled at 6, 9, 12, and 15 days after flowering, and endosperms were dissected for analysis. In proteomic study a high-resolution two-dimension gel electrophoresis and LC/MS/MS sequencing system had been established, and the protein profile for endosperms with different grain quality were also investigated. The present results showed that protein expression in TNG67 were more sensitive to high temperature than that in TN1. Among the differentially expressed proteins identified from proteomic approach, near 40 proteins were sequenced and showed highly homology to known registered enzymes or cDNA clones, including pyruvate orthophosphate dikinase, ER luminal binding protein (BiP), protein disufide isomerase, Waxy proteins, chaperone, enolase, etc. One protein (28 kDa) was closely associated with the chalky character of rice grain. In addition ,8-10 isoforms of Waxy protein were identified. Expression of 4 isoforms was positively correlated with amylose content of rice grain. The success in applying 2D gel and LC/MS/MS to link protein expression profile and grain development stages will be able to reveal key proteins involved in target physiological process and will benefit the future improvement of quality in rice. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/19829 | 其他識別: | 902313B002268 | Rights: | 國立臺灣大學農藝學系暨研究所 |
顯示於: | 農藝學系 |
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902313B002268.pdf | 2.25 MB | Adobe PDF | 檢視/開啟 |
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