Molecular study of the cold-tolerance mechanism in the root of rice (Oryza sativa L.)
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
DOI
902313B002284
Abstract
Abiotic environmental stresses, such as low
temperature frequently result in significant
reduction of the potential yield of rice.
Therefore, to understand the cold tolerance
mechanism in rice, it is essential to evaluate
and determine the genes/ or proteins
expression profile in rice under chilling stress,
and identify key transcripts or proteins that
are associated with this process. To achieve
this purpose, follows previous differential display (DDRT-PCR) approach, several
methods including PCR-selected cDNA
substraction, cDNA-AFLP and protein 2-D
gel analysis have been applied to address this
problem. The result showed that with
partial cDNA fragments derived from
DDRT-PCR using chilling tolerant (TNG 67)
or chilling sensitive (TN1) rice cultivars as
probes, several new genes have been found.
These are reversibly glycosylated polypeptide
protein (RGP), Dna-J related protein,
NADPH-dependent aldose reductase,
inorganic pyrophosphatase, Myb-like protein
and ICT. The physiological function of
these genes will be further characterized.
Also about twenty cDNA fragments that
expressed under chilling condition were
identified by PCR-selected cDNA
substraction and confirmed by reverse
Northern blot. These cDNA fragments are
now in sequencing. In addition, to look for
genes involved in the early stage of cold
signal transduction, another approach
cDNA-AFLP was carried out in this study.
So far a few cDNA fragments were obtained
and other combination primer sets will be
continuously used in searching for more
genes. Finally, a protein 2-D gel analysis
was used to reveal protein expression profiles
difference in rice roots under chilling or
re-warming condition. So far at least eight
proteins were identified. In the future,
genes or proteins identified above will be
further analysis to dissect their relationship
with cold tolerance in rice root.
Subjects
Rice
Root
Cold-tolerance
PCR
-selected cDNA substraction
-selected cDNA substraction
cDNA-AFLP
protein 2-D gel analysis
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
File(s)![Thumbnail Image]()
Loading...
Name
902313B002284.pdf
Size
427.9 KB
Format
Adobe PDF
Checksum
(MD5):aa304d578c0dbe12ac23bc212957ce3c