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  4. Molecular study of the cold-tolerance mechanism in the root of rice (Oryza sativa L.)
 
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Molecular study of the cold-tolerance mechanism in the root of rice (Oryza sativa L.)

Date Issued
2002-07-31
Date
2002-07-31
Author(s)
張孟基  
DOI
902313B002284
URI
http://ntur.lib.ntu.edu.tw//handle/246246/19832
Abstract
Abiotic environmental stresses, such as low temperature frequently result in significant reduction of the potential yield of rice. Therefore, to understand the cold tolerance mechanism in rice, it is essential to evaluate and determine the genes/ or proteins expression profile in rice under chilling stress, and identify key transcripts or proteins that are associated with this process. To achieve this purpose, follows previous differential display (DDRT-PCR) approach, several methods including PCR-selected cDNA substraction, cDNA-AFLP and protein 2-D gel analysis have been applied to address this problem. The result showed that with partial cDNA fragments derived from DDRT-PCR using chilling tolerant (TNG 67) or chilling sensitive (TN1) rice cultivars as probes, several new genes have been found. These are reversibly glycosylated polypeptide protein (RGP), Dna-J related protein, NADPH-dependent aldose reductase, inorganic pyrophosphatase, Myb-like protein and ICT. The physiological function of these genes will be further characterized. Also about twenty cDNA fragments that expressed under chilling condition were identified by PCR-selected cDNA substraction and confirmed by reverse Northern blot. These cDNA fragments are now in sequencing. In addition, to look for genes involved in the early stage of cold signal transduction, another approach cDNA-AFLP was carried out in this study. So far a few cDNA fragments were obtained and other combination primer sets will be continuously used in searching for more genes. Finally, a protein 2-D gel analysis was used to reveal protein expression profiles difference in rice roots under chilling or re-warming condition. So far at least eight proteins were identified. In the future, genes or proteins identified above will be further analysis to dissect their relationship with cold tolerance in rice root.
Subjects
Rice
Root
Cold-tolerance
PCR
-selected cDNA substraction
cDNA-AFLP
protein 2-D gel analysis
Publisher
臺北市:國立臺灣大學農藝學系暨研究所
Type
report
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