DC 欄位 | 值 | 語言 |
dc.contributor | 國立臺灣大學農藝學系暨研究所 | zh_TW |
dc.contributor.author | 張孟基 | zh_TW |
dc.creator | 張孟基 | en |
dc.date | 2002-07-31 | - |
dc.date.accessioned | 2006-07-26T01:57:09Z | - |
dc.date.accessioned | 2018-07-11T01:29:09Z | - |
dc.date.available | 2006-07-26T01:57:09Z | - |
dc.date.available | 2018-07-11T01:29:09Z | - |
dc.date.issued | 2002-07-31 | - |
dc.identifier | 902313B002284 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/19832 | - |
dc.description.abstract | 水稻經常因遭受非生物性之生理逆
境,如低溫而導致嚴重減產。因此極其重
要的是去瞭解水稻在低溫逆境下之耐冷機
制,尤其是其內部所有基因或蛋白質之表
現,找出與低溫反應相關之基因或蛋白
質。本計畫乃以水稻根部為系統,承續先
前已應用之基因差異顯示法,進一步以
PCR-selected cDNA substraction ,
cDNA-AFLP 及蛋白質雙向電泳等方法來研
究在低溫下其內部所有基因與蛋白質之表
現。實驗結果顯示,由先前基因差異顯示
法對低溫具耐性(台農67 號, TNG67) 及對
低溫敏感(台中在來一號, TN1) 之水稻根
部所得之部份cDNA 片段逕行篩庫而獲致新
增基因, 如Reversibly glycosylated
polypeptide protein (RGP), Dna-J
related protein, NADPH-dependent
aldose reductase, inorganic
pyrophosphatase, Myb-like protein 與
ICT , 將進一步分析其生理功能。在
PCR-selected cDNA substraction 之研究
上亦藉由反向北方墨氏法(revrse
Northern blot) 初步獲得近20 個在低溫
下基因表現有差異之cDNA 片段, 目前正從
事定序之進一步方析。另外在以cDNA-AFLP
之方法來尋找早期與低溫訊息傳導有關之
基因,初步得到數個在低溫下可被誘導之
cDNA 片段,將繼續就其它不同引子之組合
進行篩選。最後本計畫亦針對水稻根部在
低溫或低溫處理後予以回溫其全部蛋白質
表現群相之差異利用蛋白質雙向電泳加以
分析,目前至少找到8 個受溫度影響其表
現的蛋白質。綜合言之,本計畫未來將就
上述cDNA 片段或蛋白質,進一步分析其與
低溫反應之關係,並檢測該基因如何在生
理功能上參與低溫耐性之調控。 | zh_TW |
dc.description.abstract | Abiotic environmental stresses, such as low
temperature frequently result in significant
reduction of the potential yield of rice.
Therefore, to understand the cold tolerance
mechanism in rice, it is essential to evaluate
and determine the genes/ or proteins
expression profile in rice under chilling stress,
and identify key transcripts or proteins that
are associated with this process. To achieve
this purpose, follows previous differential display (DDRT-PCR) approach, several
methods including PCR-selected cDNA
substraction, cDNA-AFLP and protein 2-D
gel analysis have been applied to address this
problem. The result showed that with
partial cDNA fragments derived from
DDRT-PCR using chilling tolerant (TNG 67)
or chilling sensitive (TN1) rice cultivars as
probes, several new genes have been found.
These are reversibly glycosylated polypeptide
protein (RGP), Dna-J related protein,
NADPH-dependent aldose reductase,
inorganic pyrophosphatase, Myb-like protein
and ICT. The physiological function of
these genes will be further characterized.
Also about twenty cDNA fragments that
expressed under chilling condition were
identified by PCR-selected cDNA
substraction and confirmed by reverse
Northern blot. These cDNA fragments are
now in sequencing. In addition, to look for
genes involved in the early stage of cold
signal transduction, another approach
cDNA-AFLP was carried out in this study.
So far a few cDNA fragments were obtained
and other combination primer sets will be
continuously used in searching for more
genes. Finally, a protein 2-D gel analysis
was used to reveal protein expression profiles
difference in rice roots under chilling or
re-warming condition. So far at least eight
proteins were identified. In the future,
genes or proteins identified above will be
further analysis to dissect their relationship
with cold tolerance in rice root. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 438172 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學農藝學系暨研究所 | zh_TW |
dc.rights | 國立臺灣大學農藝學系暨研究所 | zh_TW |
dc.subject | 水稻 | zh_TW |
dc.subject | 根 | zh_TW |
dc.subject | 低溫耐性 | zh_TW |
dc.subject | 聚合酉母選擇
性cDNA 互補反應 | zh_TW |
dc.subject | cDNA-引子接合長度多型
性 | zh_TW |
dc.subject | 蛋白質雙向電泳 | zh_TW |
dc.subject | Rice | en |
dc.subject | Root | en |
dc.subject | Cold-tolerance | en |
dc.subject | PCR
-selected cDNA substraction | en |
dc.subject | cDNA-AFLP | en |
dc.subject | protein 2-D gel analysis | en |
dc.title | 水稻根部低溫耐性分子機制之探討 | zh_TW |
dc.title | Molecular study of the cold-tolerance mechanism in the root
of rice (Oryza sativa L.) | en |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/19832/1/902313B002284.pdf | - |
dc.coverage | 計畫年度:90;起迄日期:2001-08-01/2002-07-31 | zh_TW |
item.fulltext | with fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.grantfulltext | open | - |
crisitem.author.orcid | 0000-0001-8081-4298 | - |
顯示於: | 農藝學系
|