糯稻種原之蜡質基因DNA 序列與胚乳澱粉組成之相關性研究
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
DOI
912313B002367
Abstract
Studies on the correlations between variation in waxy gene sequences and starch components in
endosperm for glutinous-rice germplasm
To study waxy gene expression under glutinous background, to detect Wxa allele in glutinous
or japonica rice (Oryza sativa L.) varieties, to identify sequences related waxy gene expression, and
to evaluate genetic similarity among glutinous rices, the 5' upstream sequences of waxy alleles
among 144 rice germplasm, (including 118 waxy and 26 non-waxy), were analyzed. Because ten
germplasm classified as waxy-type by their appearance had higher amylose amount (>12%), there
was a little difference in judging waxy-type varieties between by checking grain appearance and
amylose content. The diverse nucleotides “G” and “T” respectively representing Wxa and Wxb were
found in glutinous germplasm. The single nucleotide substitution could explain 42.6 % of amylose
content. Besides, we have found eight alleles of (CT) repeat in our study, and six of the eight alleles
have significant effects on amylose contents. The alleles (CT)11, (CT)17 and (CT)10 contributed 34.5 %, 19.5 % and 18.7 % of total amylose variation, respectively. The finding of three glutinous
(C102, C105, and C124) and four (C010, C050, C093, and C100) japonica germplasm carrying a
Wxa allele has refreshed the previous understanding that glutinous or japonica rice carry Wxb allele
only. According to the sequence of waxy alleles, the gene tree of 144 germplasm was established.
The investigated varieties were separated predominantly by repeat numbers of (CT), amylose
content, and origins of germplasm. Similar results have been found in clustering based on ISSR
markers previously. From the GLM analysis results, 144th(G/T) and (CT) repeated alleles were
detected and other newly discovered factors including (AATT)5/6, 390-C/T, 363-T/G, and 229-G/A.
have a significant attributions for amylose accumulation. (AATT)5/6, 390-C/T, and 363-T/G could
explain 40.2 % of amylose variantion, and 229-G/A could contribute 54.9 %.
Subjects
Glutinous Rice
Germplasm
Waxy Gene
Amylose
DNA Sequence
Genetic Diversity
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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