水稻穀粒發育之研究：穀粒蛋白質表現、功能與栽培溫度之 關係﹝3/3﹞
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
DOI
922313B002002
Abstract
Proteins are essential to rice caryopsis development and quality formation. High
temperature is an important environmental factor which may decrease grain quality.
In the present study rice caryopsis proteins were profiled by two-dimensional
polyacrylamide gel electrophoresis, and differentially expressed proteins were
analyzed by liquid chromatography/tandem mass spectrometry (LC\MS\MS).
Expressions of more than 400 polypeptide spots were monitored during caryopsis
development, and more than 70 differentially expressed polypeptides were analyzed
by LC\MS\MS. We identified 54 proteins with known functions. Of these, 21 were
involved with carbohydrate metabolism, 14 with protein synthesis and sorting, and 9
with stress responses. Waxy (Wx) proteins and glutelins were the most significant
spots increased significantly during development. Allergen-like proteins, PPDK and
NADH-SDH, also were expressed during development, implying their physiological
roles in caryopsis. Expression of large isoforms of Wx proteins was correlated with
the amylose content of rice caryopses. One protein with high GC content in its DNA
sequence was correlated with the chalky trait of kernels. High temperature (35/30 ℃)
decreased expression of Wx proteins, allergen-like proteins and elongation factor 1 β,
but increased expression of small heat shock proteins (sHSP),
glyceraldehyde-3-phosphate dehydrogenase and prolamin. sHSP was positively
correlated with the appearance of chalky kernels. During development, glutelins were
phosphorylated and glycosylated, indicating these molecules were post-translationally
modified. Possible functions of the expression of candidate proteins on grain quality
are discussed.
Subjects
rice
proteomics
high temperature
grain quality
Wx protein
heat shock
protein
protein
水稻
蛋白質體學
高溫
榖粒品質
Waxy蛋白
熱休克蛋白
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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