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  4. Molecular study of the cold-tolerance mechanism in the root of rice (Oryza sativa L.)
 
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Molecular study of the cold-tolerance mechanism in the root of rice (Oryza sativa L.)

Date Issued
2004-07-31
Date
2004-07-31
Author(s)
張孟基  
DOI
922313B002006
URI
http://ntur.lib.ntu.edu.tw//handle/246246/19842
Abstract
Abiotic stresses, such as low temperature frequently result in significant reduction of the potential yield of rice. Therefore, in order to understand the cold tolerance mechanism in rice, it is essential to evaluate and determine the genes/ or proteins expression profile in rice under chilling stress, and identify key transcripts or proteins that are associated with this process. In past two years, we have identified several differentially expressed genes or proteins in response to low temperature by cDNA-AFLP and 2D gel methods. In this study, we especially focused on three genes, including ADf, porin-like protein and β-glucosidase to determine their corresponding gene copy numbers and expression patterns under various abiotic stress. After rice genome blast search, ADFs belong to a multiple gene families that consisted of 9 genes. RT-PCR analysis with 3’-specific primer pairs showed that ADF-2 and ADF-3 genes respond differentially to various abiotic stresses. In addition, the porin – like protein gene displays the property which similar to the anion channel of membrane protein. After southern blotting analysis, we found it’s a single copy gene in rice cultivars – TNG67, TN1 and Pangonia. In TNG67 root, the expression of this gene shut down when low temperature started after one hour, but it expressed again after 24 hours. Under differential abiotic stress treatment, it shows the porin-like protein gene expression repressed under heat treatment in both tissues. In order to get the full – length cDNA of beta – glucosidase gene, unexpectedly, due to the misannealing of primer pairs, we isolate another gene, which is amine oxidase. The southern blot analysis showed that there is only a single copy gene in the rice genome. After low temperature treatment, the expression of this gene was induced approximately two folds higher than the control. But after two hours, the expression reduced again. The gene may play an important role in polyamine degradation, that causes the H2O2 and NH3 accumulation. The coreelation between amine oxidase gene expression and rice cold tolerance would require further study.
Subjects
Rice
Root
Cold-tolerance
Actin
depolymerization factor (ADF)
porin-like
protein
beta-glucosidase
amine oxidase
Publisher
臺北市:國立臺灣大學農藝學系暨研究所
Type
report
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922313B002006.pdf

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