Molecular study of the cold-tolerance mechanism in the root of rice (Oryza sativa L.)
Date Issued
2004-07-31
Date
2004-07-31
Author(s)
DOI
922313B002006
Abstract
Abiotic stresses, such as low temperature
frequently result in significant reduction of
the potential yield of rice. Therefore, in order
to understand the cold tolerance mechanism
in rice, it is essential to evaluate and
determine the genes/ or proteins expression
profile in rice under chilling stress, and
identify key transcripts or proteins that are
associated with this process. In past two
years, we have identified several
differentially expressed genes or proteins in
response to low temperature by cDNA-AFLP
and 2D gel methods. In this study, we
especially focused on three genes, including
ADf, porin-like protein and β-glucosidase to
determine their corresponding gene copy
numbers and expression patterns under
various abiotic stress.
After rice genome blast search, ADFs belong to a multiple gene families that
consisted of 9 genes. RT-PCR analysis with
3’-specific primer pairs showed that ADF-2
and ADF-3 genes respond differentially to
various abiotic stresses. In addition, the
porin – like protein gene displays the
property which similar to the anion channel
of membrane protein. After southern
blotting analysis, we found it’s a single copy
gene in rice cultivars – TNG67, TN1 and
Pangonia. In TNG67 root, the expression of
this gene shut down when low temperature
started after one hour, but it expressed again
after 24 hours. Under differential abiotic
stress treatment, it shows the porin-like
protein gene expression repressed under heat
treatment in both tissues.
In order to get the full – length cDNA of
beta – glucosidase gene, unexpectedly, due to
the misannealing of primer pairs, we isolate
another gene, which is amine oxidase. The
southern blot analysis showed that there is
only a single copy gene in the rice genome.
After low temperature treatment, the
expression of this gene was induced
approximately two folds higher than the
control. But after two hours, the expression
reduced again. The gene may play an
important role in polyamine degradation,
that causes the H2O2 and NH3 accumulation.
The coreelation between amine oxidase gene
expression and rice cold tolerance would
require further study.
Subjects
Rice
Root
Cold-tolerance
Actin
depolymerization factor (ADF)
depolymerization factor (ADF)
porin-like
protein
protein
beta-glucosidase
amine oxidase
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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