https://scholars.lib.ntu.edu.tw/handle/123456789/185099
標題: | 栽培環境對稻米品質影響之蛋白質體研究:溫度調節性蛋白 質之功能分析(1/3) | 作者: | 盧虎生 | 關鍵字: | rice;proteomics;high temperature;grain quality;Wx protein;heat shock protein;水稻;蛋白質體學;高溫;稻米品質;Waxy 蛋白質;熱休克蛋白 | 公開日期: | 31-七月-2005 | 出版社: | 臺北市:國立臺灣大學農藝學系暨研究所 | 摘要: | 蛋白質為影響水稻穎果發育及稻米品質形成的主要因子,高溫環境則為主要 影響稻米品質的環境因子。本研究建立水稻穎果發育期間蛋白質表現之雙向電永 圖譜,並且利用逢機質譜分析(LC\MS\MS)的方法鑑定具表現差異的蛋白質。在表 現的蛋白質中,超過400 個於水稻穎果發育期間表現的蛋白質於雙向電泳膠中被 分離出。其中有對溫度處理會產生表現差異的蛋白質,亦有品種兼具表現差異的 蛋白質,其中利用質譜分析鑑定出具表現差異的蛋白質共有70 個,病找到其中 54 個已知功能的蛋白質。包括21 個與碳水化合物生合成與代謝相關的蛋白質, 14 個與蛋白質生合成與累積相關的蛋白質,9 個與逆境反應有關的蛋白質。Waxy (Wx)蛋白質與glutelins 為隨著水稻穎果發育期呈顯著增加的蛋白質。 Allergen-like proteins, PPDK and NADH-SDH, 也在穎果發育期間表現,且扮 演其重要的生理角色與功能。其中, Wx 蛋白質各isoforms 的表現量與amylose 的含量成正相關。此外,本研究找出一個基因,具有高度GC 比例的DNA 序列, 且可能與心腹白的形成有關。高溫(35/30 ℃)會降低Wx 蛋白質、allergen-like proteins 以及elongation factor 1 β 的表現。,但是一群低分子量的heat shock proteins (sHSP)、glyceraldehyde-3-phosphate dehydrogenase 以及prolamin 等蛋白質的表現量會增加。sHSP 的含量與心腹白的程度呈正相關。此外,本期 計畫亦利用RT-PCR 確認相關基因在RNA 層次上的表現。 Proteins are essential to rice caryopsis development and quality formation. High temperature is an important environmental factor which may decrease grain quality. In the present study rice caryopsis proteins were profiled by two-dimensional polyacrylamide gel electrophoresis, and differentially expressed proteins were analyzed by liquid chromatography/tandem mass spectrometry (LC\MS\MS). Expressions of more than 400 polypeptide spots during caryopsis development, in response to temperature treatments or between varieties were monitored. Among them, more than 70 differentially expressed polypeptides were analyzed by LC\MS\MS. We identified 54 proteins with known functions. Of these, 21 were involved with carbohydrate metabolism, 14 with protein synthesis and sorting, and 9 with stress responses. Waxy (Wx) proteins and glutelins were the most significant spots increased significantly during development. Allergen-like proteins, PPDK and NADH-SDH, also were expressed during development, implying their physiological roles in caryopsis. Expression of large isoforms of Wx proteins was correlated with the amylose content of rice caryopses. One protein with high GC content in its DNA sequence was correlated with the chalky trait of kernels. High temperature (35/30 ℃) decreased expression of Wx proteins, allergen-like proteins and elongation factor 1 β, but increased expression of small heat shock proteins (sHSP), glyceraldehyde-3-phosphate dehydrogenase and prolamin. sHSP was positively correlated with the appearance of chalky kernels. In this study, we used RT-PCR to confirm the expression profiles of those genes. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/19851 | 其他識別: | 932313B002067 | Rights: | 國立臺灣大學農藝學系暨研究所 |
顯示於: | 農藝學系 |
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932313B002067.pdf | 2.82 MB | Adobe PDF | 檢視/開啟 |
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