https://scholars.lib.ntu.edu.tw/handle/123456789/185100
標題: | 水稻營養器官中澱粉代謝基因表現之研究(I)水稻葉鞘中澱粉代謝之調控 機制(1/3) | 作者: | 王淑珍 | 公開日期: | 31-七月-2005 | 出版社: | 臺北市:國立臺灣大學農藝學系暨研究所 | 摘要: | 水稻(Oryza sativa L.)葉鞘為一暫存性組織,其在抽穗前累積的澱粉,在抽穗之後會 成為提供榖粒中碳水化合物的來源之ㄧ,此現象為水稻葉鞘sink-source 性質轉換期 間最明顯的特徵,並影響榖粒的產量。然而,控制葉鞘sink-source 轉換時期澱粉合 成及代謝之機制仍然不清楚。在我們的研究中,觀察到水稻劍葉下第二片葉片(-2 葉) 的葉鞘會在抽穗前大量累積澱粉,抽穗之後澱粉含量迅速下降。為了了解水稻 -2 葉 葉鞘澱粉在抽穗期前後含量變化的調控機制,我們利用slot-blot reverse northern 分 析的方法觀察中澱粉生合成、澱粉分解及其他醣類代謝相關酵素的基因表現。結果顯 示,ADP-glucose pyrophosphorylase large subunit (AGPL) and small subunit (AGPS), granule-bound starch synthase I (GBSSI), branching enzyme (BE) I, BE IV, soluble starch synthase (SSS) II-2, SSSIII, SSSIV-1, SSSIV-2 在抽穗前會增強表 現,抽穗之後表現量降低。而BEIII, cytosolic 及質體內 fructose-1, 6-bisphosphatase 的表現量則是在抽穗時明顯達到最高峰。澱粉分解相關酵素α-amylase 和蔗糖代謝 相關酵素sucrose phosphate synthase (SPS), invertase 及sucrose transporter (SUT)1, SUT2 則是在抽穗之後提高表現量。結果顯示這些基因可能為影響澱粉含量 在抽穗期前後變化之主要因子。藉此,我們將更進一步探討此些基因表現之調控機 制,以了解水稻葉鞘 sink-source 轉換的調控機制。 Starch accumulated in rice (Oryza sativa L.) leaf sheath before heading and degradation for providing carbon source to panicles after heading is an important factor to affect the grain yield. However, the mechanisms for controlling the starch synthesis and metabolism in leaf sheath at the sink-source transition stage are still unclear. In this study, starch content change during the heading period in the second leaf sheath (-2 leaf sheath) below flag leaf was observed. In order to understand the regulatory mechanism of starch content change during heading period, expressions of 18 genes involved in starch synthesis and degradation were identified during this period. Besides, expressions of several genes related to nitrogen metabolism, sucrose biosynthesis and photosynthesis were also determined in -2 leaf sheath. Among carbohydrate metabolism-related genes, the reverse northern hybridization data showed that the mRNA level of ADP-glucose pyrophosphorylase large subunit (AGPL) and small subunit (AGPS), granule-bound starch synthase I (GBI), branching enzyme (BE) IV, soluble starch synthase(SSS) II-2, SSSIII, SSSIV-1, SSSIV-2 were increased before heading and decreased after heading. The transcript acumulations of BEIII, cytosolic and plastidic form of frutose-1, 6-bisphosphatase were increased at the time of heading. The results were suggested these gene expressions could be related to the sink-source transition of -2 leaf sheath. On the other hand, sucrose phosphate synthase (SPS) gene expressions were more active after heading. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/19852 | 其他識別: | 932313B002070 | Rights: | 國立臺灣大學農藝學系暨研究所 |
顯示於: | 農藝學系 |
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932313B002070.pdf | 139.42 kB | Adobe PDF | 檢視/開啟 |
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