https://scholars.lib.ntu.edu.tw/handle/123456789/185102
標題: | 利用基因體技術研發特殊用途性質稻米品系(1/3) | 作者: | 盧虎生 | 關鍵字: | rice;quality breeding;proteomics;novel quality;水稻;品質育種;蛋白質體學;特殊品質 | 公開日期: | 31-七月-2005 | 出版社: | 臺北市:國立臺灣大學農藝學系暨研究所 | 摘要: | 稻作目前為我國生產面積與產值最大之農作物,自2002年加入WTO後面臨國際市場的競 爭壓力下,我國良質米的市場發展受到相當的限制。解決策略之一是研發具特殊用途品質的 品種,以開發多樣化、多用途品質的市場,增進我國稻作產業的競爭力。農業試驗所嘉義分 所近年來利用台梗9號及台農67號發展出數個高世代、已具遺傳穩定性的誘變族群,初步分析 顯示這些族群品在蛋白質、澱粉等分子上具有相當變異性,具有開發特殊性質品種的潛力。 本計畫擬以NIR方法初步篩選不同粗蛋白質與直鏈澱粉含量之品系,利用proteomics進一步分 析選拔具特殊蛋白質之品系,同時進行農藝性狀評估、田間栽培適應性評估,與利用價值評 估,期望能選出具特殊性狀且具利用潛力之新品系。本研究計畫目前已建立有效之水稻蛋白 質特性篩選方法與流程,以此方法篩選所收集之水稻品系。經篩選近330個不同性質之誘變族 群、野生種及傳統雜交組合後代高世代品系,已篩選出約30個高蛋白及30個低蛋白品系,並 由野生稻品系中發現多型性表現,及栽培稻與野生稻間的差異表現。由此些材料族群篩選出 之品系已進入今年一期作栽培試驗,將持續進行後代蛋白質表現穩定性測定及農藝性狀評 估。對於其中特殊之品系,目前正進行2D電泳及蛋白質identification分析(Q-TOF 或 LC/MS/MS), 以進一步瞭解其稻米蛋白質分子功能特性。 Rice is the largest cultivated crop on Taiwan. After entering WTO, breeding for quality improvement has been facing a bitter challenge due to importation of high quality rice from international rice market. A promising solution for the challenge is to create new varieties with novel characteristics and added market value. In recent years Agriculture Research Institute has developed several chemical induced mutation populations. These mutation populations are genetically stable and contain considerable variation in macro biomolecules content, such as proteins and amylase. In this project, we will use NIR spectroscopy to screen mutation lines having significant variation in protein and amylase contents. And then determine protein specificity and identity for selected genetic lines by proteomics. Agronomic traits of selected lines and their adaptability for organic culture system are further evaluated in the field. We have set up the effective SDS-PAGE procedure to screen mutation lines. And have screened 30 high protein and 30 low protein content lines from 330 different genetic lines, including mutation population, wild type and traditional cross-over breeding lines. In addition, there are a certain polymorphism in protein profiles between wild type lines and cultivars. The selected lines have planted for the following trials of agronomic traits and protein expression stability. Further more, we will analyze the protein function of some special lines by 2D electrophoresis and protein identification analysis. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/19854 | 其他識別: | 932317B002008 | Rights: | 國立臺灣大學農藝學系暨研究所 |
顯示於: | 農藝學系 |
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932317B002008.pdf | 250.2 kB | Adobe PDF | 檢視/開啟 |
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