https://scholars.lib.ntu.edu.tw/handle/123456789/185107
標題: | 水稻培養細胞中碳水化合物代謝之研究(2/3) Carbohydrate Metabolism in Rice Tissue Culture |
作者: | 劉麗飛 | 關鍵字: | 碳水化合物代謝;水稻;組 織培養;滲透壓逆境;carbohydrate metabolism;rice;tissue culture;osmotic stress | 公開日期: | 31-七月-1999 | 出版社: | 臺北市:國立臺灣大學農藝學系暨研究所 | 摘要: | 植物細胞具分化全能性,亦即可 經由培養條件的調整,使單一細胞再 生為完整植株。惟至目前為止,對此 分化全能性機制的了解仍極有限。本 研究室過去的研究,證明水稻的培養 細胞經滲透壓處理後,會提高細胞內 IAA、ABA 之含量,而與植株再生有 密切相關。因此,本試驗乃進一步探 討植物荷爾蒙與碳水化合物代謝的關 係,及其滲透壓誘導水稻癒合組織植 株再生過程中的角色。將台南5 號未 成熟胚培養在外加植物荷爾蒙(即同時 含有IAA 合成前驅物anthranilic acid 及 ABA 的 MSD10 培養基中(TN5- AnA)培養) 與滲透壓處理兩系統中, 結果發現:1.在癒合組織誘導階段均有 較低澱粉水解酵素活性,且主要皆因 á-Amy(á-澱粉水解酵素)蛋白質合成減 少之故。另外,在植株再生階段,兩 系統澱粉水解酵素的表現一致。2. 兩 系統澱粉代謝相關酵素的表現均類 似,但蔗糖相關代謝酵素的表現則有 明顯不同,其中在癒合組織誘導階 段,滲透壓的系統處理下蔗糖代謝相 關酵素活性均顯著被誘導,相反的, 外加植物荷爾蒙的系統處理下則SS (蔗糖合成酵素)、Sol-IT (可溶性蔗糖轉 化酵素)及Bound-IT (結合性蔗糖轉化 酵素)均顯著受到抑制。而在植株再生 階段,兩種處理下蔗糖分解酵素活性 均比對照組高。上述結果強烈顯示滲 透壓處理可能藉由內生ABA及IAA含 量的改變來調控部分碳水化合物代謝 相關酵素(如á-Amy)的表現,達到誘導 植株再生的目的。這是一全新的發 現,將可對植物分化全能性之機制有 進一步的了解。 Plant cells possess totipotency that means whole plants could be regenerated from single cell by modulating culture conditions. The mechanisms of totipotency, however, were limitedly understood so far. From thorough studies, it had found the contents of endogenous IAA and ABA were increased significantly in rice callus by osmotic stress treatment, and were correlated to the ability of shoot regeneration. We, therefore, did further studies to explore the correlation among phytohormones, carbohydrate metabolism and osmotic stress during shoot regeneration. TN5-AnA callus was induced on MSD10 basal medium containing anthranilic acid and ABA. The results showed all the phenomenons were similar to those under osmotic stress treatment. Higher IAA and ABA levels inhibited all the sucrolytic enzyme (Sol- IT, Bound-IT, and SS) and amylase activity. Therefore, the higher soluble sugars and starch contents might be resulted from sucrose uptaking by sucrose transporter dominantly and inhibiting starch degradation, respectively. In conclusion, osmotic stress induced some changes of carbohydrate metabolism, and was brought about shoot regeneration. Parts of those changes were regulated by changes of endogenous IAA and ABA levels. But there are some processes induced by osmotic stress might be regulated by other factors during shoot regeneration. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/29657 | 其他識別: | 882313B002051A06 | Rights: | 國立臺灣大學農藝學系暨研究所 |
顯示於: | 農藝學系 |
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882313B002051A06.pdf | 30.8 kB | Adobe PDF | 檢視/開啟 |
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