https://scholars.lib.ntu.edu.tw/handle/123456789/186214
標題: | Neuropathic Allodynia Involves Spinal Neurexin-1 beta-dependent Neuroligin-1/Postsynaptic Density-95/NR2B Cascade in Rats | 作者: | Lin, Tzer-Bin Lai, Cheng-Yuan Hsieh, Ming-Chun Jiang, Jian-Lin Cheng, Jen-Kun Chau, Yat-Pang Ruan, Ting Chen, Gin-Den Peng, Hsien-Yu |
公開日期: | 2015 | 卷: | 123 | 期: | 4 | 起(迄)頁: | 909-926 | 來源出版物: | Anesthesiology | 摘要: | Background: Neuroligin-1 (NL1) forms a complex with the presynaptic neurexin-1 beta (Nrx1b), regulating clustering of N-methyl-D-aspartate receptors with postsynaptic density-95 (PSD-95) to underlie learning-/memory-associated plasticity. Pain-related spinal neuroplasticity shares several common features with learning-/memory-associated plasticity. The authors thereby investigated the potential involvement of NL1-related mechanism in spinal nerve ligation (SNL)-associated allodynia. Methods: In 626 adult male Sprague-Dawley rats, the withdrawal threshold and NL1, PSD-95, phosphorylated NR2B (pNR2B) expressions, interactions, and locations in dorsal horn (L4 to L5) were compared between the sham operation and SNL groups. A recombinant Nrx1b Fc chimera (Nrx1b Fc, 10 mu g, 10 mu l, i.t., bolus), antisense small-interfering RNA targeting to NL1 (10 mu g, 10 mu l, i.t., daily for 4 days), or NR2B antagonist (Ro 25-6981; 1 mu M, 10 mu l, i.t., bolus) were administered to SNL animals to elucidate possible cascades involved. Results: SNL-induced allodynia failed to affect NL1 or PSD-95 expression. However, pNR2B expression (mean +/- SD from 13.1 +/- 2.87 to 23.1 +/- 2.52, n = 6) and coexpression of NL1-PSD-95, pNR2B-PSD-95, and NL1-total NR2B were enhanced by SNL (from 10.7 +/- 2.27 to 22.2 +/- 3.94, 11.5 +/- 2.15 to 23.8 +/- 3.32, and 8.9 +/- 1.83 to 14.9 +/- 2.27 at day 7, n = 6). Furthermore, neuron-localized pNR2B PSD-95-pNR2B double-labeled and NL1/PSD-95/pNR2B triple-labeled immunofluorescence in the ipsilateral dorsal horn was all prevented by Nrx1b Fc and NL1-targeted small-interfering RNA designed to block and prevent NL1 expression. Without affecting NL1-PSD-95 coupling, Ro 25-6981 decreased the SNL-induced PSD-95-pNR2B coprecipitation (from 18.7 +/- 1.80 to 14.7 +/- 2.36 at day 7, n = 6). Conclusion: SNL-induced allodynia, which is mediated by the spinal NL1/PSD-95/pNR2B cascade, can be prevented by blockade of transsynaptic Nrx1b-NL1 interactions. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/279391 | DOI: | 10.1097/ALN.0000000000000809 |
顯示於: | 醫學系 |
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