|Title:||Anti-Gad65 Autoantibody in Taiwanese Patients with Insulin-Dependent Diabetes Mellitus--Effect of Hla on Anti-Gad65 Positivity and Clinical Characteristics||Authors:||CHUANG, LEE-MING
|Issue Date:||1997||Source:||CLINICAL ENDOCRINOLOGY||Journal Volume:||v.47||Journal Issue:||n.4||Start page/Pages:||455-61||Abstract:||
OBJECTIVE Anti-GAD65 antibody has been studied widely in patients with insulin-dependent diabetes mellitus (IDDM) in many different populations. How- ever, the prevalence of GAD 65 autoantibody has not been assessed in Taiwanese patients with IDDM. We therefore characterized GAD65 antibody and investi- gated the effect of HLA-DR phenotypes on GAD65 autoimmunity and other clinical characteristics in Taiwanese subjects with IDDM. hundred and twenty-five patients (male 102, female 123) with IDDM were recruited. The diagnostic criteria for IDDM were age of onset before 30 years, presence of diabetic ketoacidosis, and insulin- dependency within 3 years of onset. We employed a radioligand method to detect GAD65 antibody . HLA-DR typing was performed by the PCR-SSO techniques. Plasma C-peptide and anti- thyroid microsomal antibody were also measured. GAD65 antibody according to duration of disease were 50/91 ( 54.9%), 37/95 (38.9%), 8/24 ( 33%), and 3/15 (20%) among the groups of duration ≦5, 6- 10, 11-15, and >15 years, respectively (p=0.0011). There were no significant differences between GAD(+) and GAD(-) patients in age of onset (11.5±6.5 and 11.6±13.4 years, respectively), gender distribution (male :female 39:59 and 58:69, respectively) and percentage with residual ,β cell function (38.8% and 29 .1%, respectively). Multiple regression analysis revealed that duration of IDDM correlated inverselyβ cell function. Earlier onset of IDDM correlated with a loss of β cell function and a HLA-DR phenotype containing DR3/4, DR3/3 or DR3/9. Taiwanese subjects with IDDM was negatively corrlated with duration of disease. Different determinants in the HLA-DR locus contributed to the clinical onset of IDDM but not to GAD autoimmunity.
|Appears in Collections:||醫學系|
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