https://scholars.lib.ntu.edu.tw/handle/123456789/187938
標題: | 非小細胞肺癌之微衛星體複製突變與核酸修補基因 (HMSH2基因及HOGG1基因) 發生基因變異之相關聯性(I) | 其他標題: | Microsatellite Instability and Genetic Alteratin of Two DNA Repair Genes–hMSH2 Gene and hOGG1 Gene in Non-Small Cell Lung Cancer | 作者: | 余忠仁 | 關鍵字: | 微衛星體;基因突變;核酸錯配修補;核酸氧化傷 害;hMSH2;hOGG1;肺癌;microsatellite;mutation;mismatch repair;oxidative DNA damage;lung cancer | 公開日期: | 1999 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | 基因突變現象隨著老化過程而逐步累 積於生物體中,成為癌變之潛在原因。近 來,由於發現了核酸修補系統,對於生物體 組織基因突變之調控以及修補系統基因失 常後之癌變現象,受到了廣泛的注意。微衛 星體為散佈於基因組中反覆出現之短核酸 片段,其複製變異被認為是代表遺傳因子複 製出現錯誤之標誌。在少數針對肺癌之相關 報告中,微衛星體複製突變發生率也達33-66% 。不同之微衛星體標誌於肺癌組織之突 變率,在文獻報告之差異度很大;而且至今 亦無任何有關肺癌MMR 基因變異之相關 研究報告。我們乃提出此二年期研究計劃, 目的在評估非小細胞肺癌之微衛星體複製 突變與兩個核酸修補基因:hMSH2 及 hOGG1(為新近發現,修復核酸氧化傷害之 基因),發生基因變異之相關聯性。本計劃 之第一年已收集60 對肺癌及其非肺癌肺組 織檢體,抽取DNA 及RNA ,並完成部分微 衛星體標誌變異之分析,目前正進行檢測核 酸修補基因在發生微衛星體複製變異之肺 癌檢體中之可能基因變異。 Mutation accumulates throughout life and is the underlying cause of cancer. Recently, much attention has been paid to the important role of the DNA mismatch repair system in controlling the accumulation of somatic mutations in human tissues and the association of mismatch repair deficiency with carcinogenesis. Microsatellite instability, representing mutations of short repetitive DNA sequence distributed within genomes, appears to be a novel mechanism in carcinogenesis and is thought to reflect multiple replication errors from MMR genes. Frequent (33-66%) presence of replication error phenomenon in lung cancer have been reported. There were significant differences exists in the frequency of alterations in individual marker, and data regarding the alteration of MMR genes in lung cancer are lacking. We propose a two-year study to evaluate the frequency of microsatellite instability in non-small cell lung cancer, and its association with genetic alteration of MMR genes During the first year of study, we collected 60 pairs of tissues, performed microsatellite of several markers in part of the samples, and obtained some findings. Analysis of MMR genes is undergoing. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23418 | 其他識別: | 882314B002208 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
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882314B002208.pdf | 151.77 kB | Adobe PDF | 檢視/開啟 |
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