https://scholars.lib.ntu.edu.tw/handle/123456789/187941
標題: | 人類第四型多巴胺受器在腎上腺皮質醛酮瘤的表現及其多型性的臨床意義 | 作者: | 吳寬墩 | 關鍵字: | 多巴胺受器;皮質醛酮瘤;腎上腺;多形性 | 公開日期: | 1999 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | 臨床及實驗室的證據顯示,皮質醛酮的分泌受到多巴胺的抑制。雖然這種調節可能 是經由類似第二型的多巴胺受器(dopamine receptor, DR )的作用,但是並無研究證明 是何種的DR 。目前已知類似第二型的多巴胺受器至少有三種: D2, D3, D4 。為探討是何 種DR 存在腎上腺的皮質,吾人以逆轉化-多聚脢鏈鎖反應法(RT-PCR ),和原位雜交 (in situ hybridization ),來檢視腎上腺以及其腫瘤。吾人發現D2 和D4 表現在腎上腺 的皮質和髓質;且兩者的分布並無明顯差異。在皮質的表現兩者主要在小球區(zona glomerulosa ),少部份在網狀區(zona reticularis ),但束狀區(zona fasciculata )則幾 無訊號。D3 無論在皮質或髓直皆無表現。 D2 和D4 表現於嗜鉻細胞瘤(pheochromocytoma ),且兩者在分布及量並無明顯差異。 雖然這兩種受器也存在皮質醛酮瘤(aldosterone-producing adenoma, APA ),但D2 的表 現並不一致,有些APA 並不表現D2 。反之,D4 在所有的腎上腺和APA 皆有明顯的表 現。D4 和D2 在APA 的表現主要是在類非束狀區細胞(nonzona fasciculata-like cells ), 這個發現符合吾人過去認為,APA 細胞的類非束狀區細胞可能表現較多的D2-like 受 器。總之,吾人的這項研究結果,可能是第一次發現D2 和D4 ,尤其是D4 ,表現在人 類的腎上腺皮質,特別是在小球區。嗜鉻細胞瘤同時表現D2 和D4 。但在APA 中,D4 的表現較明顯且一致,雖然,部份APA 也有D2 的表現。而D4 的表現主要在類非束狀 區細胞。 The aldosterone secretion is evidently regulated by a dopaminergic inhibitory mechanism. Pharmacological characterization and autoradiographic studies revealed a D2-like receptor in the adrenal cortex, especially in the zona glomerulosa (ZG). However, the subtype of the dopamine receptors (DR) involving this regulation is not elucidated, although a D2-like receptor was suspected. To investigate which subtype of DR expresses in the adrenal cortex, we examined the messages of D2-like receptors, D2, D3 and D4, by RT-PCR and in situ hybridization of adrenal glands and adrenal neoplasm. Both D2 and D4 receptors were expressed in normal adrenal gland, pheochromocytoma and aldosterone-producing adenoma (APA). However, D2 receptor was not universally expressed in all adrenal samples in contrast with D4 receptor that was detected in all cases of APA and adrenal remnant. No D3 message was detected by RT-PCR in all adrenal samples. In the adrenal cortex, the messages of D2 and D4 were mainly localized in the ZG and, to a lesser extent, in the zona reticularis. In APA, the non-zona fasciculata-like cells expressed D4 and D2 receptors more abundantly than zona fasciculata-like cells. In summary, we demonstrated the existence of both D2 and D4 receptors in the human adrenal gland and adrenal neoplasm. The expression of both DR in the adrenal cortex is mainly localized in the ZG. This observation indicates that both DR may play a role in the regulation of aldosterone secretion. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23421 | 其他識別: | 882314B002234 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
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