https://scholars.lib.ntu.edu.tw/handle/123456789/188003
標題: | 飲食治療對高膽固醇血症誘發之血管內皮細胞通透性變化之影響 | 其他標題: | The Effect of Diet Therapy on the Hypercholestrolemia-induced Vascular Permeability Change in Rabbit Iris | 作者: | 吳造中 | 關鍵字: | 血管內皮通透性;高膽固醇血症;飲食治療;螢光光度測定儀;眼虹彩;Vascular permeability;hypercholesterolemia;diet;interendothelial junction | 公開日期: | 2001 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | 近年來,血管內皮細胞功能失全,由於會導致多種疾病而引起廣泛的注意。過 去的研究顯示血管內皮乃動脈管腔中物質通過或沉積到血管壁上的一個屏障,動脈血管 壁通透性的增加會導致或加速動脈硬化瘢的形成。在心血管系疾病的危險因子中,高膽 固醇血症可說是眾所皆知與動脈粥狀硬化性疾病相當有關的重要危險因子。已有許多研 究顯示高膽固醇血症會促使血管內皮功能失全。過去也已有大型的研究顯示要治療或預 防動脈硬化性心血管疾病時應包含一完善的飲食治療,且單單飲食治療便可確實降低此 疾病的罹病率及死亡率。然而,由於方法之缺乏,至今仍鮮少有研究探討飲食治療是否 可以改變血管通透性,其有關之功能及病理組織形態學變化之詳細序列研究亦付闕如。 在這一年的研究計劃中,我們使用序列的眼部前房螢光光度計測量法來探討飲 食治療對以高膽固醇血症所誘發之血管內皮通透性變化之影響。我們將對60 隻紐西蘭 白兔,餵食12 週的高膽固醇飲食(0.5%cholesterol-enriched diet )誘發高膽固醇 血症後,改以一般正常飲食餵食80 週,來降低其血中膽固醇值;同時我們亦以一般兔 飼料來餵食另60 隻的紐西蘭白兔共92 週 (對照組)。這兩組兔子我們均在基礎時及其 後每兩週測量其眼部的血水屏障功能變化、血中膽固醇及三酸甘油酯濃度、及眼虹彩部 粥狀硬化瘢之出現及消失狀況。我們比較血中脂質濃度、血管通透度及虹彩部粥狀硬化 瘢之序列變化,並找出其在飲食治療後的變化排序及其相關性。我們發現,血管通透性 在粥狀硬化的相當早期便已發生且可為飲食治療來改善。形態檢查發現此功能變化與細胞間隙的改變有關。 To study the effect of diet therapy on the vascular permeability, serial anterior chamber fluorophotometric examinations were carried out on 60 controls (group I) and 60 diet-induced hypercholesterolemic (group II) rabbits. Functional and morphopathological changes of the blood-aqueous barrier associated with total serum cholesterol (CHO) levels were studied during the 12 weeks of standard (group I) or 0.5% cholesterol-enriched diet (group II) feeding followed by 80 weeks of standard rabbit chow feeding schedule. The blood-aqueous barrier (F60 value) in group II was more permeable than that in group I since the second week (group I: 491.0+35.9 ng/ml, group II: 868.4+85.7 ng/ml, respectively, p<0.0001). The F60 escalated further up to the 22nd week; thereafter it declined slowly to its baseline at 32nd week of the experiment. The rise and decrease of F60 was relatively parallel to, however, lagged behind the change of CHO level. In group I, most of the interendothelial junctions of the iridic microvasculature were interdigitating type. In contrast, many of the interendothelial junctions became not only the overlap type but also the end-to-end typed or even open junction after 12 weeks of lipid-enriched diet feeding in group II. These endothelial changes in the hyperlipidemic animals gradually recovered while the CHO level decreased after the diet therapy. In conclusion, the vascular permeability change occurred in the very early stage of atherosclerosis and could be reversed by the diet therapy. This functional and morphological study clarified how hypercholesterolemia increased the vascular permeability. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23484 | 其他識別: | 892314B002410 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
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892314B002410.pdf | 56.85 kB | Adobe PDF | 檢視/開啟 |
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