https://scholars.lib.ntu.edu.tw/handle/123456789/188221
標題: | Real-Time Pcr for Rapid Genotyping of Angiotensin-Converting Enzyme Insertion/Deletion Polymorphism | 作者: | TSENG, CHIN-HSIAO | 關鍵字: | angiotensin-converting enzyme;real-time PCR;insertion/ deletion polymorphism;DELETION POLYMORPHISM;HEART-DISEASE;ACE GENE | 公開日期: | 2001 | 卷: | v.34 | 期: | n.8 | 起(迄)頁: | 661-666 | 來源出版物: | CLINICAL BIOCHEMISTRY | 摘要: | Objective: To develop a real-time PCR technique for detection of the insertion/deletion (YD) polymorphism of angiotensin-converting enzyme (ACE ) gene.Design and methods: Three primers were designed for performing real -time PCR in the presence of SYBR Green I as flurochrome followed by melting curve analysis. Forty human genomic DNA that have been genotyped by two-rounds of conventional PCR were used for evaluation of this technique.Results: Melting curve analysis indicated the melting peak at 73 .9degreesC and 76.2 degreesC corresponding to the presence of I and D alleles, respectively. Comparable genotyping results were obtained by both conventional and real-time PCR. Besides, the mistyping of ID allele individuals by the first run of conventional PCR were accurately genotyped by single-tube real time PCR. Conclusions: The real-time PCR method presented in this study provides a rapid and sensitive way for genotyping of ACE gene that may be suitable for large-scale clinical and epidemiologic study. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95168 |
顯示於: | 醫學系 |
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