https://scholars.lib.ntu.edu.tw/handle/123456789/188268
標題: | Correlation between Serum Lipid Profiles and the Ratio and Count of the Cd16+Monocyte Subset in Peripheral Blood of Apparently Healthy Adults | 作者: | HUANG, ZEI-SHUNG | 關鍵字: | CD16;HDL-cholestcrol;LDL-cholesterol;monocytes;atherosclerosis;HIGH-DENSITY-LIPOPROTEIN | 公開日期: | 2002 | 卷: | v.101 | 期: | n.1 | 起(迄)頁: | 11-17 | 來源出版物: | JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION | 摘要: | Background and Purpose: Deposited vascular oxidized low- density lipoproteins (LDLs) are important triggers of the transformation of circulatory monocytes into macrophages. CD 16+ monocytes have been reported to be the precursors of tissue macrophages, In this study, we sought to determine the relationship between serum LDL-cholesterol and the percentage and count of the CD16+ monocyte subset in the peripheral blood of healthy adults. Methods: We studied the correlations between serum lipid profiles and both peripheral CD16+ and CD36+ monocyte subset ratios and counts in apparently healthy adults (50 men and 50 women). Monocyte surface antigens CD16 and CD36 on CD14+ monocytes were detected using fluorescent triple staining and flow- cytometry. Surface staining was performed by incubating 1 x 10(6) blood mononuclear cells with phycoerythrin-conjugated anti-CD14, fluorescein isothiocyanate-conjugated anti-CD36, and respective control isotopes (mouse IgGs). A total of 5, 000 cells were counted and the frequency of surface antigens was determined by FACscan. Results: A significant positive link between LDL-cholesterol and the CD16+ subset ratio was found by linear correlation analysis (p < 0 .05) but not by multivariate regression analysis. Both linear correlation analysis and ANOVA, revealed a significant inverse link between high- density lipoprotein (HDL)-cholesterol and the CD16+ subset ratio (both p<0 .01). By multivariate regression analysis, gender was the main 0 significant determinant for the CD16+ subset ratio. When serum total cholesterol (TC) was excluded from the analysis to avoid the interference from collinearity between serum TC and LDL (r = 0.84), HDL- cholesterol became independently and inversely linked to the CD16+ subset ratio. There were independent inverse links between HDL-cholesterol and the counts of all monocytes, CD 16+, monocytes, and CD36+ monocytes. Conclusions: Our results suggest that circulating HDL-cholesterol may be much more important than LDL-cholesterol in affecting the transformation of circulatory monocytes into macrophages. The inverse link between HDL- cholesterol and the number of macrophage precursors in peripheral blood might contribute partly to the well-known antiatherogenic effect of HDL- cholesterol. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95216 |
顯示於: | 醫學系 |
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