Disabled-2 Small Interfering Rna Modulates Cellular Adhesive Function and Mapk Activity during Megakaryocytic Differentiation of K562 Cells
Resource
FEBS LETTERS v.541 n.1-3 pp.21-27
Journal
FEBS LETTERS
Journal Volume
v.541
Journal Issue
n.1-3
Pages
21-27
Date Issued
2003
Date
2003
Author(s)
Tseng, Ching-Ping
Huang, Chien-Ling
Huang, Ching-Hui
Cheng, Ju-Chien
Stern, Arnold
Tseng, Chin-Hsiao
Abstract
Previous studies have shown that Disabled-2 (DAB2) is up- regulated during megakaryocytic differentiation of human K 562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell-cell adhesion and mitogen-activated protein kinase ( MAPK) phosphorylation. The morphological changes and 03 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129-135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression.
Subjects
disabled-2
small interfering RNA
K562 cell
megakaryocytic differentiation
mitogen-activated protein kinase
Type
journal article