https://scholars.lib.ntu.edu.tw/handle/123456789/188297
Title: | Disabled-2 Small Interfering Rna Modulates Cellular Adhesive Function and Mapk Activity during Megakaryocytic Differentiation of K562 Cells | Authors: | Tseng, Ching-Ping Huang, Chien-Ling Huang, Ching-Hui Cheng, Ju-Chien Stern, Arnold Tseng, Chin-Hsiao |
Keywords: | disabled-2;small interfering RNA;K562 cell;megakaryocytic differentiation;mitogen-activated protein kinase | Issue Date: | 2003 | Journal Volume: | v.541 | Journal Issue: | n.1-3 | Start page/Pages: | 21-27 | Source: | FEBS LETTERS | Abstract: | Previous studies have shown that Disabled-2 (DAB2) is up- regulated during megakaryocytic differentiation of human K 562 cells. To delineate the consequences of DAB2 induction, a DNA vector-based small interfering RNA (siRNA) was designed to intervene in DAB2 expression. We found that DAB2 siRNA specifically inhibited DAB2 induction, resulting in the modulation of cell-cell adhesion and mitogen-activated protein kinase ( MAPK) phosphorylation. The morphological changes and 03 integrin expression associated with megakaryocytic differentiation were not affected. Since the MAPK pathway has been shown to involve DAB2 induction [Tseng et al., Biochem. Biophys. Res. Commun. 285 (2001) 129-135], our results suggest a reciprocal regulation between DAB2 and MAPK in the differentiation of K562 cells. In addition, we have demonstrated for the first time that DAB2 siRNA is a valuable tool for unveiling the biological consequences of DAB2 expression. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/95246 |
Appears in Collections: | 醫學系 |
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