https://scholars.lib.ntu.edu.tw/handle/123456789/188333
標題: | 以螢光二次元差異性電泳及質譜儀來分析肝癌與其相對應的非癌肝組織,蛋白質表現的差異 | 作者: | 許金川 | 關鍵字: | hepatocellular carcinoma;proteomics;differential gel electrophoresis;DIGE;MALDI;mass spectrometry | 公開日期: | 2004 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | Hepatocellular carcinoma (HCC) has been the leading cause of cancer death in Taiwan. About 6000-8000 people died of this cancer every year in Taiwan. Though regular sonographic examination can early detect small HCC and there are many therapeutic modalities for HCC, the therapeutic results remains unsatisfactory. To improve the survival, further investigation of the early diagnostic markers and the mechanisms of hepatocarcinogenesis is very important. In the recent years, investigating the genome-wide expression profiles of cancers has been the predominant method to identify cancer-related genes. Though using cDNA microarray for genome-wide expression profiling is a very powerful tool to clarify the genetic changes in cancers, the major pitfall of these methods is that the mRNA expression does not parallel protein expression in many cases. The introduction of fluorescent 2D differential gel electrophoresis (DIGE) has now made it possible to detect and quantitate differences between experimental pairs of samples resolved on the same 2D gel. The basis of this technique is to use two fluorescent dyes (Cy3 and Cy5) to differentially label lysine residues of two protein samples for comparative analysis on a single gel. The ability to directly compare two samples on the same gel not only avoids the complications of gel-to-gel variation but also enables a more accurate and rapid analysis of differences and reduces the number of gels that need to be run. Following automated image analysis, using the novel and innovative software, spots of interest are selected for gel excision, subjected to in-gel enzymatic digestion, and mass spectrometry identification. In this current project, we enrolled eight paired of HCC and the corresponding non-tumor liver tissues, and subjected to DIGE analysis. We found that 9 proteins (eg. heat shock protein) were upregulated in the HCC tissues, while 11 proteins were downregulated in the HCC tissues. We are currently to investigate whether these proteins could be used as the new HCC diagnostic markers. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23636 | 其他識別: | 922314B002143 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
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922314B002143.pdf | 625.46 kB | Adobe PDF | 檢視/開啟 |
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