https://scholars.lib.ntu.edu.tw/handle/123456789/188343
標題: | 研究anti-ribosomal P抗體之臨床價值與是否由黴菌感染所致 | 作者: | 陳茂源 | 關鍵字: | 全身性紅斑狼瘡;Anti-ribosomal P 抗體;黴菌ribosomal P2 蛋白;SLE;Anti-ribosomal P2;Fungal ribosomal proteins | 公開日期: | 2004 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | 全身性紅斑狼瘡以有多樣自體免疫抗體著稱.Anti-ribosomal P 抗體是其中之一.過去的 抗體陽性率調查曾有作者指出海外華人之紅斑狼瘡患者有較高之陽性率,約為36 至38%. 我們以送來做anti-dsDNA 檢驗之血清做人類ribosomal P2 自體免疫抗體的西方墨點檢驗 , 發現有38.6%之血清呈陽性反應.我們更進一步去測試anti-ribosomal P 抗體是否因感染 黴菌經由交叉反應而來的假設.因為黴菌或原蟲與eukaryotic 細胞之ribosomal P 蛋白類 似,部分氨基酸列序幾乎一致.由於Anti-ribosomal P 抗體之抗原決定部位集中於蛋白之 carboxyl 尾端之22 個胺基酸,我們以黴菌之ribosomal P 蛋白非尾端部分為抗原,測試 紅斑狼瘡病患是否有對抗黴菌蛋白之抗體 .我 們從P.marneiffei 及 C.neoformans 拿 到 P2 之cDNA,去掉C 尾端之13 個胺基酸,以此作為抗原.在全部血清中有26.9%對其中 一種或兩種黴菌抗原有反應.有人類ribosomal P2 自體免疫抗體的血清也是28.6%有黴菌 蛋白抗體 .此一結果雖不支持交叉反應 ,但 亦未完全排除 .因 對有P2 自體免疫抗體的病 患而言,carboxyl 尾端之22 個胺基酸有可能是最強的抗原,在抗體成熟的過程中,其他 抗體轉弱而消失. The anti-ribosomal P antibodies may have clinical association with psychosis, nephritis,hepatitis and disease activity in patients with systemic lupus erythematosus.It is interesting that oversea Chinese lupus patients have higher prevalence of this antibody than white and black patients.We study the prevalence of anti-ribosomal P antibody in sera sent for detection of anti-dsDNA.Among the 145 sera,the positive rate is 38.6%by western blot.Next,we test the theory that anti-ribosomal P autoantibodies are induced by fungal infection due to cross-reaction because the carboxyl terminal is conserved between fungus and eukaryotic cells.The cDNA and PCR product of fungal ribosomal P2 protein were obtained from P.marneiffei and C.neoformans by RT-PCR.The carboxyl terminal 13 amino acids were deleted and PCR product was cloned into expressing vector.26.9% of the 145 sera have reaction to one or both of the truncated fungal P2 protein. Sera with P2 autoantibodies have similar positive rate of 28.6%.This result does not support the theory of cross-reaction.However,cross-reaction can still be the cause of autoimmunity if C terminus is the major epitope to patients with P2 autoantibodies.Antibodies to other epitopes of low affinity may be lost during the maturation of humoral immunity. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23646 | 其他識別: | 922314B002178 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
檔案 | 描述 | 大小 | 格式 | |
---|---|---|---|---|
922314B002178.pdf | 32.02 kB | Adobe PDF | 檢視/開啟 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。