https://scholars.lib.ntu.edu.tw/handle/123456789/188407
標題: | 以SELDI來尋找人類肝細胞癌的生物標記 | 作者: | 許金川 | 公開日期: | 2005 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | Although the significant risk factors for hepatocellular carcinoma (HCC) are well known from epidemiological studies, diagnosis of this disease at an early stage is difficult and HCC remains one of the leading causes of cancer death worldwide. Thus, to identify any useful HCC-related biomarkers is still a need. We performed surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify differentially expressed proteins in HCC serum using weak cation exchange (WCX2) protein chips. Protein characterization was performed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation and nano flow liquid chromatography tandem mass spectrometry (nano LC MS/MS). A total of fifty-six sera were collected from HCC patients and compared with forty-eight patients with chronic hepatitis and nine healthy individuals. A candidate marker with molecular weight about 8900 Da was detected as differentially expressed in patients with chronic hepatitis C and hepatitis C virus-related HCC. We identified this differentially expressed protein as complement C3a. The expression of C3a in HCC sera was further validated by PS20 chip immunoassay and Western blotting. Complement C3a was found to be elevated in patients with chronic hepatitis C and HCV-related HCC. Combination of SELDI-TOF MS and 2D-PAGE provide a solution to identify disease-associated serum biomarkers. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/23712 | 其他識別: | 932314B002227 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
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932314B002227.pdf | 486.74 kB | Adobe PDF | 檢視/開啟 |
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