https://scholars.lib.ntu.edu.tw/handle/123456789/188416
標題: | 國人肺腺癌之基礎與臨床研究─建立體外模式研究肺腺癌轉移之機轉(III) | 作者: | 楊泮池 | 關鍵字: | 肺腺癌;轉移機轉;Lung adenocarcinoma;metastasis | 公開日期: | 1999 | 出版社: | 臺北市:國立臺灣大學醫學院內科 | 摘要: | 本子計劃擬做為此整合型計劃之核心 計劃,收集病人之臨床資料、血液,建立 國人肺腺癌之細胞株及收集肺腺癌組織, 包括組織冷凍保存、石蠟包埋及OCT 冷凍 組織,收集正常及肺癌RNA 及DNA ,並 建立免疫組織染色技術,提供子計劃一、 二、三、五、六、七、八應用,我們也擬 建立肺腺癌轉移之體外模式,提供其他各 子計劃之研究材料,本計畫已建立肺腺癌 細胞之高侵襲及轉移傾向和低侵襲及轉移 傾向之各類腺癌細胞株(Am J Respir Cell Mol Biol 1997 ),用之研究細胞型態學、 細胞表面受體、細胞粘附分子、細胞表面 蛋白及分解酵素、細胞移動因子、轉移相 關基因等之改變。利用子計畫七建立之多 色cDNA 微陳列技術,及我們已建立之肺 癌細胞轉移模式,分析與肺癌轉移之相關 基因,初步我們已獲得數十個基因,其分 別表現於高侵襲轉移能力的細胞株,或低 侵襲轉移能力的細胞株,在北方墨點技術 也同時證實其相關性,目前我們正分析及 序定這些可能基因轉移機轉,其中我們選植 CRMP-1, 並轉植入CL1-5,目前正研究其 功能及在肺癌轉移機轉所扮演之可能角色。 Lung cancer is a serious health problem in Taiwan. The epidemiologic characteristics such as: high female to male ratio, high proportion of adenocarcioma, high peripheral type lung cancer which are rather unique and may imply that different etiologic factors involving the pathogenesis of lung cancer in Taiwan. This proposed project will address on the most common cell type of lung cancer: adenocarcinoma, especially female adenocarcinoma and study the molecular epidemiology, genetic alterations and mechanism of metastasis. The subproject 4 will serve partially as a core facilities. We will provide clinical informations, collect tissue samples, for extraction of DNA and RNA, blood, establish cell lines from our own Chinese patients for the studies in other subprojects. We also provide immunohisto-chemical technical support for other collaborated laboratories. We will particularly focus on the establishment an in vitro model for study the mechanisms of metastasis in lung adenocarcinoma, by repeated selection in Transwell Membrane Culture System, we establish high invasive and high metastatic potential sublines. Using these in vitro cell model, we are able to explore the genes involving the regulation of cancer metastasis by the technique of differential display of gene expressions as well as the cDNA microarray technique Several condidate cDNAs have been obtained which are differentially expressed either in high invasive metastatic cell line or in low invasive/metastatic cell line. Northern hybridization also confirmed the results obtained from cDNA microarray. We cloned and transfected one candidate gene-CRMP-1 into CL1-5. Currently we are involved in characterization function of these genes. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/29760 | 其他識別: | 882314B002079M39 | Rights: | 國立臺灣大學醫學院內科 |
顯示於: | 醫學系 |
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882314B002079M39.pdf | 257.57 kB | Adobe PDF | 檢視/開啟 |
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