|Title:||國人肺腺癌之基礎與臨床研究─黏液素蛋白表現與肺癌之關係||Authors:||余忠仁||Keywords:||GMucin glycoprotein;mucin gene;monoclonal antibody;polyclonal antibody;lung cancer||Issue Date:||1999||Publisher:||臺北市：國立臺灣大學醫學院內科||Abstract:||
Aberrant expression of mucins and
mucin related antigens are noted to be poor
survival factors in cancer. Experimentally,
mucins had been demonstrated to promote
tumor cell invasion, metastasis and modulate the immune recognition
phenomenon of cancer cells. Only few
studies have addressed the prognostic
implications of mucin and mucin associated
antigens in lung cancer. Our previous
investigations had discovered the
association among lung adenocarcinoma,
sialomucin and Muc5AC expression.
Therefore, to study the regulation of
Muc5AC protein expression should be an
important topic in investigating lung cancer.
In order to study the protein expression of
Muc5AC in lung cancer, 20 kD fusion
protein of Muc5AC nontandem repeat
sequence was made, injected into animals
(chicken, and mice), and successfully
generated anti-Muc5AC polyclonal and
monoclonal antibodies. These antibodies
were applied in the immunohistochemistry,
western blotting of variable specimens of
lung cancer, as well as ELISA assay. The
results are unsatisfactory.
|Appears in Collections:||醫學系|
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