DC 欄位 | 值 | 語言 |
dc.contributor.author | 李啟明 | zh_TW |
dc.creator | 李啟明 | zh_TW |
dc.date | 2002 | zh_TW |
dc.date.accessioned | 2006-08-31T05:53:10Z | - |
dc.date.accessioned | 2018-07-11T05:39:04Z | - |
dc.date.available | 2006-08-31T05:53:10Z | - |
dc.date.available | 2018-07-11T05:39:04Z | - |
dc.date.issued | 2002 | - |
dc.identifier | 902320B002162M52 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/29783 | - |
dc.description.abstract | 氧化低密度脂蛋白(ox-LDL)是誘發血
管內皮細胞功能失調及動脈粥狀硬化之重
要原因之一。以往認為氧化密度脂蛋白經
巨噬細胞的清道夫受器攝取後才引發內皮
細胞病變。然而經實驗顯示,在缺乏巨噬
細胞的狀態下,氧化密度脂蛋白 能直接作
用於內皮細胞而引發功能失全。近來血管
內皮細胞被發現本身具有一種清道夫受器
- 內皮細胞氧化低密度脂蛋白受體
(LOX-1) ,能攝取氧化密度脂蛋白。LOX-1
由一個類似lectin 的胞外分域、胞膜穿透分
域、以及胞內分域三者所構成。到目前為
止, LOX-1 和ox-LDL 結合後對內皮細胞
中蛋白質活性及基因表現的影響還不是很
清楚。本計畫目的在瞭解ox-LDL 誘發血管
內皮細胞功能失調的分子機制。我們以
LOX-1 為餌,藉由酵母菌雙雜交法(Yeast
two hybrid screen),從人類心臟cDNA
library 中釣到15 個會和LOX-1 交互作用的
基因。 更進一步,我們藉由微陣列分析
(microarray analysis),從人類的臍靜脈內皮
細胞(HUVEC)中,找到39 個基因,其表現
會因ox-LDL 的刺激而改變。找出這些基
因,將有助於我們瞭解ox-LDL 對血管內皮
細胞功能的影響,更進一步對治療動脈硬
化疾病的新要開發有所助益。 | zh_TW |
dc.description.abstract | Oxidative modification of low-density
lipoprotein (ox-LDL) is crucial in the
pathogenesis of endothelial dysfunction,
which is a major characteristic of
atherosclerosis. Similar to that of
macrophage, vascular endothelial cells can
internalize ox-LDL. The recognition and
uptake of ox-LDL in endothelial cells is
different from that of macrophage in that it is
mediated by LOX-1, a C-type lectin-like
scavenger receptor. To date, the
physiological effects of LOX-1 on protein
activities and gene expression levels after it
binds ox-LDL and internalizes into
endothelial cells are still unclear. Our aim for
this project was to investigate the molecular
mechanism of endothelial cell dysfunction
caused by ox-LDL. We first did yeast
two-hybrid screen using the N-terminal
domain of LOX-1 (LOX-1N) as the bait.
Fifteen genes whose protein products interact
with LOX-1N were cloned from a human
heart cDNA library. Furthermore, we did
microarray analysis and found in HUVEC
cells 39 genes whose expression levels were
changed after ox-LDL stimulation.
Identification of these genes will help us
understand the molecular effect of ox-LDL
on endothelial cell function and discover new
drugs for the treatments of atherosclerosis. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 52557 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院內科 | zh_TW |
dc.rights | 國立臺灣大學醫學院內科 | zh_TW |
dc.subject | 氧化低密度脂蛋白 | zh_TW |
dc.subject | 內皮細胞功
能失調 | zh_TW |
dc.subject | 動脈粥狀硬化 | zh_TW |
dc.subject | 內皮細胞氧化低
密度脂蛋白受體 | zh_TW |
dc.subject | ox-LDL | en |
dc.subject | endothelial dysfunction | en |
dc.subject | atherosclerosis | en |
dc.subject | LOX-1 | en |
dc.title | 氧化低密度脂蛋白誘發動脈粥狀硬化之致病機轉─內皮細胞氧化低密度脂蛋白受體LOX-1之訊息傳遞機制(3/3) | zh_TW |
dc.title.alternative | Oxidized LDL signaling through the endothelial receptor- LOX-1 (3/3) | en |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/29783/1/902320B002162M52.pdf | - |
dc.coverage | 計畫年度:90;起迄日期:2001-08-01/2002-07-31 | zh_TW |
item.openairetype | report | - |
item.fulltext | with fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.grantfulltext | open | - |
item.languageiso639-1 | zh_TW | - |
item.cerifentitytype | Publications | - |
顯示於: | 醫學系
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