|Title:||Securin Depletion Sensitizes Human Colon Cancer Cells to Fisetin-Induced Apoptosis||Authors:||楊培銘
|Keywords:||Fisetin;Securin;p53;Human colon cancer cells||Issue Date:||2011||Start page/Pages:||96-104||Source:||Cancer Letters||Abstract:||
Securin is highly-expressed in various tumors including those of the colon In this study the role of securin in the anticancer effects of fisetin on human colon cancer cells was investigated Fisetin-induced apoptosis in HCT116 cells as indicated by TUNEL assay Annexin V-FITC/PI double staining Ser15-phosphorylation of p53 and cleavages of procaspase-3 and PARP These effects were enhanced in HCT116 securin-null cells or in wild-type cells in which securin was knockdown by siRNA but attenuated when wild-type or non- degradable securin was reconstituted Moreover fisetin did not induce apoptosis in HCT116 p53-null and HT-29 p53-mutant cells Knockdown of securin in HCT116 p53-null cells potentiated fisetin- induced cytotoxicity by induction of apoptosis Our results provide the first evidence to support that securin depletion sensitizes human colon cancer cells to fisetin-induced apoptosis.
|Appears in Collections:||醫學系|
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