|Title:||Klebsiella pneumoniae Peptidoglycan-Associated Lipoprotein and Murein Lipoprotein Contribute to Serum Resistance, Antiphagocytosis, and Proinflammatory Cytokine Stimulation||Authors:||Hsieh, Pei-Fang
|Keywords:||Klebsiella pneumoniae;outer membrane proteins;peptidoglycan-associated lipoprotein;murein lipoprotein;outer membrane protein A||Issue Date:||2013||Start page/Pages:||1580-1589||Source:||The Journal of Infectious Diseases||Abstract:||
Background. Peptidoglycan-associated lipoprotein (Pal), murein lipoprotein (LppA), and outer membrane protein A (OmpA) are dominant outer membrane proteins (OMPs) that are released by gram-negative bacteria during sepsis. OMPs are implicated in the maintenance of cell envelope integrity. Here, we characterize the roles of these OMPs in pathogenesis during bacteremia caused by Klebsiella pneumoniae.
Methods. pal-, lppA-, and ompA-deficient K. pneumoniae strains were constructed using an unmarked deletion method. Serum sensitivity, antiphagocytosis activity, outer membrane permeability, and sensitivity to anionic detergents and antimicrobial polypeptides were determined for these OMP gene deletion mutants. The ability of these OMP gene deletion mutants to induce immune responses was compared with that of the wild-type strain in a bacteremic mouse model.
Results. Klebsiella pneumoniae strains deleted for pal or lppA exhibited reduced protection from serum killing and phagocytosis; perturbation to the outer membrane permeability barrier and hypersensitivity to bile salts and sodium dodecyl sulfate. The strain mutated for lppA had reduced ability to activate Toll-like receptor 4. Immunization of mice with the pal or lppA mutant provided protection against infection by the wild-type strain.
Conclusions. Our findings indicate that K. pneumoniae Pal and LppA proteins are important in the maintenance of cell integrity, contribute to virulence, and could be used as attenuated vaccines.
bile salt; cefotaxime; ciprofloxacin; colistin; detergent; dodecyl sulfate sodium; gentamicin; immunoglobulin G; interleukin 6; murein lipoprotein; outer membrane protein; outer membrane protein A; peptidoglycan associated lipoprotein; polymyxin B; polypeptide antibiotic agent; toll like receptor 4; unclassified drug; animal experiment; animal model; antibiotic sensitivity; article; bacterial outer membrane; bacterial strain; bacterial virulence; bactericidal activity; controlled study; cytokine production; disease model; female; gene deletion; gene mutation; human; human cell; immune response; infection prevention; infection resistance; innate immunity; Klebsiella pneumoniae; Klebsiella pneumoniae infection; membrane permeability; minimum inhibitory concentration; mouse; nonhuman; pathogenesis; permeability barrier; phagocytosis; priority journal; receptor upregulation; wild type; Klebsiella pneumoniae; murein lipoprotein; outer membrane protein A; outer membrane proteins; peptidoglycan-associated lipoprotein; Animals; Anti-Bacterial Agents; Bacterial Outer Membrane Proteins; Cell Membrane Permeability; Cytokines; Detergents; Disease Models, Animal; Humans; Inflammation Mediators; Klebsiella Infections; Klebsiella pneumoniae; Mice; Microbial Sensitivity Tests; Mutation; Neutrophils; Peptidoglycan; Phagocytosis; Phenotype; Virulence
|Appears in Collections:||醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.