https://scholars.lib.ntu.edu.tw/handle/123456789/191961
Title: | Suppression of Elevated Plasma Interleukin-8 Levels Due to Total Ischemia and Reperfusion of the Small Intestine by Luminal Perfusion with Fetal Bovine Serum | Authors: | CHEN, YUN HUNG, WEN-TSUNG CHEN, SHIH-MING TSENG, SHENG-HONG |
Keywords: | fetal bovine serum;interleukin-8;total ischemia;reperfusion | Issue Date: | 2002 | Journal Volume: | v.18 | Journal Issue: | n.2-3 | Start page/Pages: | 107-109 | Source: | PEDIATRIC SURGERY INTERNATIONAL | Abstract: | A previous study demonstrated that continuous enteric luminal perfusion of fetal bovine serum (FBS) protects the small intestine from total ischemia/reperfusion injury (IRI) and increases the intestinal mass. In this study, we further investigated the changes in plasma interleukin-8 ( IL -8) level caused by total ischemia/reperfusion of the small intestine and the effect of FBS on plasma IL-8 levels. A 3-h total ischemia was induced in a 15-cm segment of terminal ileum and then reperfusion was instituted. Luminal perfusion of FBS was conducted via an osmotic minipump connected to the stomach through a fine polyethylene tube, starting 3 days prior to total ischemia. The rats were killed after 10 and 30 min and 1 and 3 h of total ischemia, and 1, 6, and 12 h or 1, 2, and 3 days after initiation of reperfusion. Plasma IL-8 was measured by enzyme-linked immunosorbent assay. The results were compared among the FBS-treated and untreated groups. The plasma IL-8 level was elevated from 1 h of total ischemia to 6 h after initiation of reperfusion ( P< 0.05) with a peak of 641.5 +/- 36.9 pg/ml in the untreated group and 471.6 +/- 42.2 pg/ml in the treated group. Luminal perfusion of FBS significantly suppressed plasma IL-8 levels after 1 h of total ischemia and 1 h after initiation of reperfusion ( P< 0.05). The results suggest that FBS might play a role in the treatment of total IRI of the small intestine. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/91566 |
Appears in Collections: | 醫學系 |
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