https://scholars.lib.ntu.edu.tw/handle/123456789/192095
Title: | 細胞骨骼蛋白與擴張性心肌病變 Cytoskeleton and Heart Failure |
Authors: | 朱樹勳 | Keywords: | 擴張性心肌病變;蛋白質基因組分析;質譜儀;二維膠質電泳;dilated cardiomyopathy;proteomics;tandem mass spectrometry;twodimensional gel electrophoresis | Issue Date: | 31-Jul-2000 | Publisher: | 臺北市:國立臺灣大學醫學院外科 | Abstract: | 心臟血管疾病的病因皆相當複雜,用傳統的生化與遺傳的方法研究,通常是事倍 功半。我們嘗試用蛋白基因體分析的方式來研究擴張性心肌病變(dilated cardiomyopathy; DCM) 作研究,以鑑別出與DCM 相關的蛋白質與蛋白質修飾基。我們使 用差別性萃取法(Differential extraction)純化心肌纖維蛋白後,使用二維膠質電泳 (Two-dimensional gel electrophoresis)分離這些蛋白質,再用質譜儀作心肌纖維蛋白質 的鑑定與修飾基的研究。舉mlc-2 蛋白為例,我們發現一病人之Ser-14 有3.7% 被磷 酸化,另外兩病人則都有近17%的磷酸化。此項差異在臨床上之重要性,則尚待進一步 的探討,尤其樣本數必須增加,以利統計學之比較分析。 Cardiovascular diseases, like dilated cardiomyopathy (DCM), are usually very complex and the elucidation of pathogenic mechanisms is very difficult. Dilated cardiomyopathy (DCM) is a severe heart disease leading to heart insufficiency and most heart transplantations are indicated by DCM. We employed proteomics methods to analyze myocardial proteins derived from DCM myocardium, particularly myofibril proteins. We successfully isolated myofibril proteins using differential extraction method and then resolved them on a 2D gel electrophoresis system. The identities of proteins are verified using liquid chromatographytandem mass spectrometry and specific modifications on these proteins can also be identified. Using myosin regulatory light chain 2 (mlc-2) as an example, we demonstrated that how modification is mapped and quantitatively determined using selected ion tracing approach. One patient in our reseach has 3.7% phosphorylation at Ser-14, while the other two both have a phosphorylation percentage of ~16%. The significance of this difference will be validated as more samples are analyzed. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/24411 | Other Identifiers: | 892314B002190 | Rights: | 國立臺灣大學醫學院外科 |
Appears in Collections: | 醫學系 |
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892314B002190.pdf | 416.27 kB | Adobe PDF | View/Open |
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