DC Field | Value | Language |
dc.contributor | 國立臺灣大學醫學院外科 | zh_TW |
dc.contributor.author | 朱樹勳 | zh_TW |
dc.creator | 朱樹勳 | zh_TW |
dc.date | 2000-07-31 | - |
dc.date.accessioned | 2006-07-26T03:40:03Z | - |
dc.date.accessioned | 2018-07-11T09:21:07Z | - |
dc.date.available | 2006-07-26T03:40:03Z | - |
dc.date.available | 2018-07-11T09:21:07Z | - |
dc.date.issued | 2000-07-31 | - |
dc.identifier | 892314B002190 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/24411 | - |
dc.description.abstract | 心臟血管疾病的病因皆相當複雜,用傳統的生化與遺傳的方法研究,通常是事倍
功半。我們嘗試用蛋白基因體分析的方式來研究擴張性心肌病變(dilated
cardiomyopathy; DCM) 作研究,以鑑別出與DCM 相關的蛋白質與蛋白質修飾基。我們使
用差別性萃取法(Differential extraction)純化心肌纖維蛋白後,使用二維膠質電泳
(Two-dimensional gel electrophoresis)分離這些蛋白質,再用質譜儀作心肌纖維蛋白質
的鑑定與修飾基的研究。舉mlc-2 蛋白為例,我們發現一病人之Ser-14 有3.7% 被磷
酸化,另外兩病人則都有近17%的磷酸化。此項差異在臨床上之重要性,則尚待進一步
的探討,尤其樣本數必須增加,以利統計學之比較分析。 | zh_TW |
dc.description.abstract | Cardiovascular diseases, like dilated cardiomyopathy (DCM), are usually very complex
and the elucidation of pathogenic mechanisms is very difficult. Dilated cardiomyopathy
(DCM) is a severe heart disease leading to heart insufficiency and most heart transplantations
are indicated by DCM. We employed proteomics methods to analyze myocardial proteins
derived from DCM myocardium, particularly myofibril proteins. We successfully isolated
myofibril proteins using differential extraction method and then resolved them on a 2D gel
electrophoresis system. The identities of proteins are verified using liquid chromatographytandem
mass spectrometry and specific modifications on these proteins can also be identified.
Using myosin regulatory light chain 2 (mlc-2) as an example, we demonstrated that how
modification is mapped and quantitatively determined using selected ion tracing approach.
One patient in our reseach has 3.7% phosphorylation at Ser-14, while the other two both have
a phosphorylation percentage of ~16%. The significance of this difference will be validated as
more samples are analyzed. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 426257 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院外科 | zh_TW |
dc.rights | 國立臺灣大學醫學院外科 | zh_TW |
dc.subject | 擴張性心肌病變 | zh_TW |
dc.subject | 蛋白質基因組分析 | zh_TW |
dc.subject | 質譜儀 | zh_TW |
dc.subject | 二維膠質電泳 | zh_TW |
dc.subject | dilated cardiomyopathy | en |
dc.subject | proteomics | en |
dc.subject | tandem mass spectrometry | en |
dc.subject | twodimensional
gel electrophoresis | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | 細胞骨骼蛋白與擴張性心肌病變 | zh_TW |
dc.title | Cytoskeleton and Heart Failure | en |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/24411/1/892314B002190.pdf | - |
dc.coverage | 計畫年度:89
第一期;起迄日期:1999-08-01/2000-07-31 | zh_TW |
item.languageiso639-1 | zh_TW | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
item.openairetype | report | - |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.cerifentitytype | Publications | - |
Appears in Collections: | 醫學系
|