https://scholars.lib.ntu.edu.tw/handle/123456789/192158
DC Field | Value | Language |
---|---|---|
dc.contributor | 外科 | en |
dc.contributor.author | CHEN, JIN-CHERNG | en |
dc.contributor.author | CHEN, YUN | en |
dc.contributor.author | SU, YEN-HAO | en |
dc.contributor.author | TSENG, SHENG-HONG | en |
dc.creator | 陳金城;陳芸;蘇彥豪;曾勝弘 | zh-tw |
dc.creator | CHEN, JIN-CHERNG;CHEN, YUN;SU, YEN-HAO;TSENG, SHENG-HONG | en |
dc.date | 2007 | en |
dc.date.accessioned | 2008-12-15T18:00:45Z | - |
dc.date.accessioned | 2018-07-11T09:22:08Z | - |
dc.date.available | 2008-12-15T18:00:45Z | - |
dc.date.available | 2018-07-11T09:22:08Z | - |
dc.date.issued | 2007 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/91825 | - |
dc.description.abstract | Background: Celecoxib, a cyclooxygenase-2 inhibitor has been found to inhibit the proliferation of several kinds of cancer cells: however, the effects of celecoxib on glioma cells are not clear. Materials and Methods: A172 glioma cells were treated with various concentrations of celecoxib for 4, 24 or 48 h. Cytotoxic drug effects were studied by MTT (3-[4,5- dimethylthiazole-2-yl]-2,5- diphenyltetrazolium bromide)-based colorimetric assay, and celecoxib-induced apoptosis of glioma cells was investigated by FACScan. Western blot analysis was used to study celecoxib effects on the expression of mitogen-activated protein kinases (MAPKs) , p53, p21, 14-3-4 sigma, Bcl-2 and Bax. Caspace-3 activity in glioma cells was analyzed by caspase activity assay. Results: Celecoxib exerted cytotoxic effects upon and induced apoptosis of the A172 glioma cells in a concentration and time-dependent manner (p<0.05). Celecoxib had no effects on expression of MAPKs, Bax, or p21; however, it increased expression of p53 and 14-3-4 sigma, and reduced expression of Bcl-2. Celecoxib also increased the activity of caspace-3 in glioma cells. The apoptotic fraction of A 172 cells induced by 24-h treatment with 100 mu M celecoxib was reduced from 39% to 23% by pretreatment wi. th caspace-3 inhibitor (DEVD-CHO) (p<0.001). Conclusion: The results suggest that celecoxib induced cytotoxicity and apoptosis in this line of glioma cells and that such effects might be related to activation of p53 and 14-3-3 sigma, reduced Bcl-2 and Bcl-2/Bax ratio, and increased caspace-3 activiy. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | ANTICANCER RESEARCH v.27 n.4B pp.2547-2554 | en |
dc.relation.ispartof | ANTICANCER RESEARCH | - |
dc.subject | apoptosis | en |
dc.subject | celecoxib | en |
dc.subject | cytotoxicity | en |
dc.subject | glioma cells | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | Celecoxib Increased Expression of 14-3-3 Sigma and Induced Apoptosis of Glioma Cells | en |
dc.type | journal article | en |
dc.relation.pages | 2547-2554 | - |
dc.relation.journalvolume | v.27 | - |
dc.relation.journalissue | n.4B | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
Appears in Collections: | 醫學系 |
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