https://scholars.lib.ntu.edu.tw/handle/123456789/192158
Title: | Celecoxib Increased Expression of 14-3-3 Sigma and Induced Apoptosis of Glioma Cells | Authors: | CHEN, JIN-CHERNG CHEN, YUN SU, YEN-HAO TSENG, SHENG-HONG |
Keywords: | apoptosis;celecoxib;cytotoxicity;glioma cells | Issue Date: | 2007 | Journal Volume: | v.27 | Journal Issue: | n.4B | Start page/Pages: | 2547-2554 | Source: | ANTICANCER RESEARCH | Abstract: | Background: Celecoxib, a cyclooxygenase-2 inhibitor has been found to inhibit the proliferation of several kinds of cancer cells: however, the effects of celecoxib on glioma cells are not clear. Materials and Methods: A172 glioma cells were treated with various concentrations of celecoxib for 4, 24 or 48 h. Cytotoxic drug effects were studied by MTT (3-[4,5- dimethylthiazole-2-yl]-2,5- diphenyltetrazolium bromide)-based colorimetric assay, and celecoxib-induced apoptosis of glioma cells was investigated by FACScan. Western blot analysis was used to study celecoxib effects on the expression of mitogen-activated protein kinases (MAPKs) , p53, p21, 14-3-4 sigma, Bcl-2 and Bax. Caspace-3 activity in glioma cells was analyzed by caspase activity assay. Results: Celecoxib exerted cytotoxic effects upon and induced apoptosis of the A172 glioma cells in a concentration and time-dependent manner (p<0.05). Celecoxib had no effects on expression of MAPKs, Bax, or p21; however, it increased expression of p53 and 14-3-4 sigma, and reduced expression of Bcl-2. Celecoxib also increased the activity of caspace-3 in glioma cells. The apoptotic fraction of A 172 cells induced by 24-h treatment with 100 mu M celecoxib was reduced from 39% to 23% by pretreatment wi. th caspace-3 inhibitor (DEVD-CHO) (p<0.001). Conclusion: The results suggest that celecoxib induced cytotoxicity and apoptosis in this line of glioma cells and that such effects might be related to activation of p53 and 14-3-3 sigma, reduced Bcl-2 and Bcl-2/Bax ratio, and increased caspace-3 activiy. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/91825 |
Appears in Collections: | 醫學系 |
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