基因微陣列分析缺血/灌流對人類肝切除手術引發之相關細胞凋亡基因表現之研究(2/2)
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
李伯皇
DOI
912314B002161
Abstract
Ischemia is known to be a cause of
apoptosis and atrophy in experimental
animals. Until today, the exact mechanisms
and mediators involved in ischemia /
reperfusion injury remains unclear. In our
previous study on rats, liver was ischemia 60
minutes followed by reperfusion for 24 hours.
Results showed that the death of hepatocytes
is maximal after reperfusion for 6 hours by
TUNEL assay and clear DNA ladder bands.
Whereas the expressions of bax RNA, bcl-x
RNA, and bcl-xL were not negative at the
end of ischemia, which imply the regulated
apoptosis related genes might have been
expressed at this time. Furthermore, we made
a study to recognize the effect of intermittent
ischemia following reperfusion for 6 hours
induction apoptosis on rats' liver, and the
continuous ischemia was compared. The
finding showed that the injury of intermittent
ischemia was more serious than that of
continuous ischemia after reperfusion for 6
hours. And the longer time of intermittent
ischemia, the more hepatocytes were
damaged. The expression of bax, bcl-xL and
fas all were positive.
We strongly believe it is worth to
investigate the molecular mechanisms of
induced apoptosis by ischemia / reperfusion
during liver resection operations in human
patients. So we made a study to test the
effects of continuous and intermittent
ischemia on induction of hepatocytes death during liver resection in human patients. In
addition, we investigated the expression of
related apoptotic genes by cDNA microarray.
Therefore the focus of our project was
exploring objectively and correctly the injury
of intermittent vs. continuous ischemia on
liver surgery in human patients, and the
expression of apoptosis-related genes which
makes induction of damage or protective
effects during early state.
We designed the experiments on liver
resection operations of 10 liver cancer
patients in National Taiwan University
Hospital. The liver specimens and serum
were collected at the time points of 0, 15 and
30 minutes in ischemia condition, and
following the reperfusion time points of 5, 15
and 30 minutes. We extracted the RNA of
liver specimens and then performed the
cDNA microarray experiments which used
the 9600 -clones chips. After statistic
analysis, we found out some genes
significantly different from those expressed
at the control time point, ischemia 0 minutes.
There were 13 different expression genes in
period of ischemia and 31 genes in period of
reperfusion. Among them 5 genes were
overlapping.
Subjects
ischemia
reperfusion
cDNA
microarray
microarray
liver
apoptosis
SDGs
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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