比較出生後不同時期心臟基因的表現

Abstract

在產後心臟的發育期間控制細胞增生及分化的分子機制了解依舊有限。在這研究中,我們用 cDNA 微陣列調查在老鼠心肌衰弱發育期間有差異表達的基因。微陣列( 6,144 個基因)以呈色 系統用以研究老鼠從 0 天,初生7 天, 14 天, 到成年老鼠心肌中差異表現的基因。根據產後老 鼠心肌細胞的成熟度,有66 個基因被抑制並且有51 個基因被活化。這些基因包括與細胞週期, 生長激素,訊息傳遞,新陳代謝,細胞骨架,細胞外間質,和一些未知功能的基因。微陣列的資料提 供心肌發育期間基因表現模式的一個全視野的輪廓。這個資訊更進一步探索心肌發育的遺傳 控制和對各種心肌衰弱等疾病中分子機制將很有助益。

The molecular mechanisms controlling the cardiomyocyte proliferation and differentiation during postnatal development are poorly understood. In this study, we investigate the differentially expressed genes during mouse myocardial development in a genome-wide scale by cDNA microarray technique. The mouse cDNA microarray (6,144 genes) with colorimetric detection system was used to identify the differentially expressed genes in the mouse myocardium from 0-day neonatal, 7-day, 14-day postnatal, to adult ICR mice. According to the maturity of postnatal mice, the mRNA expression levels of 66 genes are down-regulated and 51 genes are up-regulated. Our data demonstrated that these genes related to cell cycle, growth factors, signal transduction, metabolic enzymes, cytoskeletone/extracellular matrix proteins, and some expressed sequence tag genes may play the roles in the postnatal myocardial growth. Identification of the differentially expressed genes between neonatal (synthetic) and adult (contractile) myocytes by microarray provides a global profiling of gene expression pattern during myocardial development. This information will be very helpful for further exploring the genetic control of cardiomyocyte proliferation and understanding the molecular mechanisms of cardiomyocytes loss and regeneration in various myocardial diseases.