比較出生後不同時期心臟基因的表現
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
林芳郁
DOI
912314B002228
Abstract
The molecular mechanisms controlling the cardiomyocyte proliferation and differentiation during
postnatal development are poorly understood. In this study, we investigate the differentially
expressed genes during mouse myocardial development in a genome-wide scale by cDNA
microarray technique. The mouse cDNA microarray (6,144 genes) with colorimetric detection
system was used to identify the differentially expressed genes in the mouse myocardium from 0-day
neonatal, 7-day, 14-day postnatal, to adult ICR mice. According to the maturity of postnatal mice,
the mRNA expression levels of 66 genes are down-regulated and 51 genes are up-regulated. Our
data demonstrated that these genes related to cell cycle, growth factors, signal transduction,
metabolic enzymes, cytoskeletone/extracellular matrix proteins, and some expressed sequence tag
genes may play the roles in the postnatal myocardial growth. Identification of the differentially
expressed genes between neonatal (synthetic) and adult (contractile) myocytes by microarray
provides a global profiling of gene expression pattern during myocardial development. This
information will be very helpful for further exploring the genetic control of cardiomyocyte
proliferation and understanding the molecular mechanisms of cardiomyocytes loss and regeneration
in various myocardial diseases.
Subjects
cardiomyocyte
postnatal development
gene expression
microarray
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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