遠端約制 / 誘導在老鼠心臟之保護作用

Abstract

Background: Internal organ remote preconditioning (RPC), such as kidney and mesentery artery, had been proved to have protective effect for myocardial infarction, but it is not feasible in clinical status. This study was performed to evaluate skeletal RPC protection in acute myocardial infarction model. Methods and Results: RPC was performed by repeated 4-cycle 10-min ischemia-reperfusion of femoral artery. The coronary artery was occluded for 2 hours (time C) to produce infarction 2 hour after RCP (time B). Four groups experiment was designed: I, sham group, without RPC and infarction; II, RPC only; III, infarction only; IV, incorporating both RPC and infarction. The infarct size was significantly reduced for group IV (22.7 ± 7.0%) compared to group III (51.6 ± 8.2%) (p<0.0001). The data pertaining to cardiac enzymes (creatine kinase and troponin I) also revealed significant decrease in the level for group IV compared to group III at time C. Western blotting of heat shock protein (HSP) revealed that consistent elevation of HSP 25 and 70 in group II, III and IV. Antioxidant enzyme in the myocardium at the area of risk revealed that Mn-superoxidase dismutase and glutathione peroxidase were consistently elevated with HSP data, but not for Cu/Zn-superoxidase dismutase and catalase. This finding suggested that RPC had protective effect via heat shock protein and partial antioxidant enzyme. Conclusions: The skeletal RPC can produce a protective effect of myocardial infarction that may be applied in clinical setting to limit the myocardial damage when infarction occurs.