A Clinically Feasible Protection “Outside the Box” in Acute Myocardial Infarction Model – Skeletal remote preconditioning
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
Chen, Yih-Sharng
DOI
912314B002232
Abstract
Background:
Internal organ remote preconditioning (RPC), such as kidney and mesentery artery, had
been proved to have protective effect for myocardial infarction, but it is not feasible in
clinical status. This study was performed to evaluate skeletal RPC protection in acute
myocardial infarction model.
Methods and Results:
RPC was performed by repeated 4-cycle 10-min ischemia-reperfusion of femoral artery.
The coronary artery was occluded for 2 hours (time C) to produce infarction 2 hour after
RCP (time B). Four groups experiment was designed: I, sham group, without RPC and
infarction; II, RPC only; III, infarction only; IV, incorporating both RPC and infarction.
The infarct size was significantly reduced for group IV (22.7 ± 7.0%) compared to group
III (51.6 ± 8.2%) (p<0.0001). The data pertaining to cardiac enzymes (creatine kinase and
troponin I) also revealed significant decrease in the level for group IV compared to group
III at time C. Western blotting of heat shock protein (HSP) revealed that consistent
elevation of HSP 25 and 70 in group II, III and IV. Antioxidant enzyme in the
myocardium at the area of risk revealed that Mn-superoxidase dismutase and glutathione
peroxidase were consistently elevated with HSP data, but not for Cu/Zn-superoxidase
dismutase and catalase. This finding suggested that RPC had protective effect via heat
shock protein and partial antioxidant enzyme.
Conclusions:
The skeletal RPC can produce a protective effect of myocardial infarction that may be
applied in clinical setting to limit the myocardial damage when infarction occurs.
Subjects
Myocardial infarction
muscle
protein
remote preconditioning
infarct size
antioxidant
enzyme
enzyme
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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