DC 欄位 | 值 | 語言 |
dc.contributor | 國立臺灣大學醫學院外科 | zh_TW |
dc.contributor.author | 陳維昭 | zh_TW |
dc.creator | 陳維昭 | zh_TW |
dc.date | 2003-07-31 | - |
dc.date.accessioned | 2006-07-26T03:41:53Z | - |
dc.date.accessioned | 2018-07-11T09:26:50Z | - |
dc.date.available | 2006-07-26T03:41:53Z | - |
dc.date.available | 2018-07-11T09:26:50Z | - |
dc.date.issued | 2003-07-31 | - |
dc.identifier | 912314B002329 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/24506 | - |
dc.description.abstract | 本研究以燒燙傷小鼠的動物模式來探討glutamine (GLN)之添加對燒燙傷小鼠血
中T 淋巴球分佈及特異性抗體產生之影響。由於燒燙傷病患最常被綠膿桿菌
(Pseudomonas aeruginosa)感染,本研究以綠膿桿菌疫苗在燒傷前注入,來研究燒
傷前後不同時間點特異性綠膿桿菌抗體產生之情形。實驗以剛斷奶之雄性
BALB/c mice 為對象,將小鼠分成2 組,一組以酪蛋白為蛋白質來源,另一組則
以4﹪(w/w)的GLN 取代酪蛋白,兩組除胺基酸組成不同外,其餘飼料組成均完
全相同。實驗開始前先眼窩採血當成測量抗體之基準值,並將綠膿桿菌疫苗(PEIF)
注入小鼠背部皮下,在第四週時給予第二次疫苗注射,實驗期共7 週。在第七週
結束時將小鼠引致30%燒傷,並於燒傷後24 小時犧牲取血,測定T 淋巴球分布
情形,及各不同時間點抗體產生之情形。結果顯示在T 淋巴球分布方面,不論
是CD3, CD4, CD8 在兩組之間均無差異。特異性綠膿桿菌抗體之產生,則在燒
傷前的各時間點及燒傷後GLN 添加組均顯著較控制組高。此結果顯示GLN 之
添加有促進體液性免疫反應之作用,但CD4 T 細胞之增生可能不是特異性抗體
產生之原因。 | zh_TW |
dc.description.abstract | This study investigated the effect of glutamine (GLN) supplementation on
specific antibody production and T lymphocyte distribution in burned mice vaccinated
with detoxified Pseudomonas exotoxin A linked with the outer membrane proteins I
and F, named PEIF. Fifty BALB/c mice were assigned to 2 groups. One group was
fed a control diet with casein as the protein source, while the other group was
supplemented with 4% GLN replaced part of casein. The 2 groups were
isonitrogenous. The mice were immunized twice with PEIF, and the production of
specific antibodies against PEIF was measured every week. After 8 weeks, all mice
received a 30% body surface area burn injury. Mice were sacrificed 24h after the
burn. The distribution of blood T lymphocyte subpopulations and the specific
antibody production were analyzed. The results demonstrated that there were no
significant differences in CD3, CD4 and CD8 T cell populations between the 2 groups
after the burn. The production of specific antibodies against P. aeruginosa
significantly increased in the GLN group at 4 and 7 weeks after immunization, and 24
h after the burn. These results suggest that vaccinating mice with GLN
supplementation may enhance humoral immunity before and after burn injury. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 27722 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學醫學院外科 | zh_TW |
dc.rights | 國立臺灣大學醫學院外科 | zh_TW |
dc.subject | 燒傷 | zh_TW |
dc.subject | glutamine | zh_TW |
dc.subject | 淋巴球分佈 | zh_TW |
dc.subject | 特異性抗體 | zh_TW |
dc.subject | 疫苗 | zh_TW |
dc.subject | 綠膿桿菌 | zh_TW |
dc.subject | burn | en |
dc.subject | T cell population | en |
dc.subject | specific antibody | en |
dc.subject | vaccination | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | L-glutamine添加對燒燙傷老鼠營養素代謝及免疫反應之影響 (II) | zh_TW |
dc.title | Effects of L-glutamine supplementation on nutrient metabolism and immune response
in thermal injured mice (II) | en |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/24506/1/912314B002329.pdf | - |
dc.coverage | 計畫年度:91;起迄日期:2002-08-01/2003-07-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
顯示於: | 醫學系
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