The preparation of new collagen layer on PU sur face and the effect on the growth of endothelial cells
Date Issued
2001-07-31
Date
2001-07-31
Author(s)
王水深
DOI
892314B002310M08
Abstract
Surface endothelization of PU or PTFE
is an important way to reduce thrombosis and
enhance its blood compatibility. This project
tried to photochemically graft GRGD peptide
to PU-PEG surface and plasma treat PTFE
membrane with C2H2/N2 for endothelization
of those materials. The results slowed that
GRGD peptide were successfully grafted to
PU-PEG surface confirmed by analysis of
ATR-FTIR and ESCA. Moreover, MTT tests
for cell metabolism showed that the higher the
peptide concentration grafted, the more the
cells adhered and proliferated on the matrix.
In regards to plasma treatment on PTFE
membrane, surface N/C and F/C ratios were
two times increases and six times decreases
respectively, that indicated successful the modifications of PTFE surface. Moreover,
post-treatment PTFE membrane could adhere
and proliferate endothelial cells confirmed by
photograph and MTT tests while nontreatment
one was not good for cell adhesion.
In conclusion, surface modification with
peptide for PU-PEG film and C2H2/N2 plasma
treatment for PTFE membrane could enhance
endothelial cells adhesion and proliferation.
Subjects
PU
UV chemical reaction
PTFE
Plasma
Endothelial cell
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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