https://scholars.lib.ntu.edu.tw/handle/123456789/192543
標題: | Shear Stress-Induced Aggregation of Oxidized Platelets | 作者: | WANG, SHOEI-SHEN | 關鍵字: | oxidation stress of platelets;the expression of GpIb receptors;SIOPA;VON-WILLEBRAND-FACTOR;HYDROXYL RADICALS;HYDROGEN-PEROXIDE | 公開日期: | 2002 | 卷: | v.105 | 期: | n.4 | 起(迄)頁: | 325-329 | 來源出版物: | THROMBOSIS RESEARCH | 摘要: | Although shear stress-induced platelet aggregation (SIPA) has been noted, the shear stress-induced aggregation of oxidized platelets (SIOPA) has not been investigated. To investigate SIOPA, small quantity of hemoglobin (Hb) Solution was first added to plasma to induce oxidation stress of platelets and then the plasma was sheared by a cone/plate viscometer at shear stress of 66 dyn/cm(2). In addition, to investigate the change in expression of the membrane receptors of glycoprotein lb (Gplb) for oxidized platelets, mean fluorescence intensity (MFI) for those platelets was detected by flow cytometric technique. The results showed that the level of the oxidation stress of platelets, as presented in malondialdehyde (MDA) values, was well correlated with the quantities of added Hb. Moreover, the aggregation of SIOPA corresponded with the levels of oxidation stress of platelets. Platelets pretreated with aspirin could only partially reduce the aggregation of SIOPA . Similarly, aspirin pretreatment could partially reduce the lowering of MFI value that indicated downexpression of Gplb receptors. Furthermore, the aggregations of SIOPA corresponded with the lowered percentage of those MF1 values for studied cases. We thereby suggested that the aggregation of SIOPA is related to both the level of oxidation stress and the downexpression of Gplb receptors for oxidized platelets. (C) 2002 Elsevier Science Ltd. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/96245 |
顯示於: | 醫學系 |
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