https://scholars.lib.ntu.edu.tw/handle/123456789/192624
Title: | Fetal Bovine Serum Suppresses Apoptosis in the Small Intestine after Total Ischemia and Reperfusion in Mice | Authors: | HUNG, WEN-TSUNG CHEN, YUN TSENG, SHENG-HONG |
Keywords: | bcl-2;p53;bax;apoptosis;fetal bovine serum;ischemia/ reperfusion | Issue Date: | 2004 | Journal Volume: | v.39 | Journal Issue: | n.7 | Start page/Pages: | 1077-1083 | Source: | JOURNAL OF PEDIATRIC SURGERY | Abstract: | Background/Purpose: Total ischemia/reperfusion (I/R) of the small intestine induces cellular apoptosis. In this study, the authors investigated the effects of fetal bovine serum ( FBS) on apoptosis and related proapoptotic and antiapoptotic factors in the I/R-injured small intestine of mice. Methods: The mice underwent total I/R of the small intestine and were treated with oral gavages of normal saline or F for 3 days, concluding with total ischemia of the small intestine. Samples of the I/R-injured small intestine, representing various predefined time- points post- I/R (immediately before and after 1 hour of total ischemia and at 1, 6, and 24 hours after initiation of reperfusion), were subjected to terminal deoxynucleotidyl transferase- mediated dUTP nick-end labeling (TUNEL) staining to study the cellular apoptosis, and Western blot analysis to measure expression of p53, bcl- 2 and bax, with caspase- activity assay used to determine the activity of caspase 3. Results: For the saline-treated mice, increased cellular apoptosis, suppressed expression of p53 and bcl-2 (with decreased bcl-2 to bax ratio) and increased caspase-3 activity were found for the I/R-injured small intestine. By contrast, FIBS treatment suppressed cellular apoptosis, producing upregulation of p53 and bcl-2 (with increased bcl-2 to bax ratio to 5.4-fold of the saline- treated group) and inhibiting the activity of caspase 3 (P<. 05). Conclusions: The results of our study suggest that I/R - induced apoptosis of the mouse small intestine may be related to p53 and bcl- 2 suppression (with decreased bcl-2 to bax ratio) and activation of caspase 3 and that such apoptosis seems to be suppressed by FBS treatment. (C) 2004 Elsevier Inc. All rights reserved. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/96326 |
Appears in Collections: | 醫學系 |
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