https://scholars.lib.ntu.edu.tw/handle/123456789/192624
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 外科 | en |
dc.contributor.author | HUNG, WEN-TSUNG | en |
dc.contributor.author | CHEN, YUN | en |
dc.contributor.author | TSENG, SHENG-HONG | en |
dc.creator | 洪文宗;陳芸;曾勝弘 | zh_TW |
dc.creator | HUNG, WEN-TSUNG;CHEN, YUN;TSENG, SHENG-HONG | en |
dc.date | 2004 | en |
dc.date.accessioned | 2009-01-08T06:24:30Z | - |
dc.date.accessioned | 2018-07-11T09:36:35Z | - |
dc.date.available | 2009-01-08T06:24:30Z | - |
dc.date.available | 2018-07-11T09:36:35Z | - |
dc.date.issued | 2004 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/96326 | - |
dc.description.abstract | Background/Purpose: Total ischemia/reperfusion (I/R) of the small intestine induces cellular apoptosis. In this study, the authors investigated the effects of fetal bovine serum ( FBS) on apoptosis and related proapoptotic and antiapoptotic factors in the I/R-injured small intestine of mice. Methods: The mice underwent total I/R of the small intestine and were treated with oral gavages of normal saline or F for 3 days, concluding with total ischemia of the small intestine. Samples of the I/R-injured small intestine, representing various predefined time- points post- I/R (immediately before and after 1 hour of total ischemia and at 1, 6, and 24 hours after initiation of reperfusion), were subjected to terminal deoxynucleotidyl transferase- mediated dUTP nick-end labeling (TUNEL) staining to study the cellular apoptosis, and Western blot analysis to measure expression of p53, bcl- 2 and bax, with caspase- activity assay used to determine the activity of caspase 3. Results: For the saline-treated mice, increased cellular apoptosis, suppressed expression of p53 and bcl-2 (with decreased bcl-2 to bax ratio) and increased caspase-3 activity were found for the I/R-injured small intestine. By contrast, FIBS treatment suppressed cellular apoptosis, producing upregulation of p53 and bcl-2 (with increased bcl-2 to bax ratio to 5.4-fold of the saline- treated group) and inhibiting the activity of caspase 3 (P<. 05). Conclusions: The results of our study suggest that I/R - induced apoptosis of the mouse small intestine may be related to p53 and bcl- 2 suppression (with decreased bcl-2 to bax ratio) and activation of caspase 3 and that such apoptosis seems to be suppressed by FBS treatment. (C) 2004 Elsevier Inc. All rights reserved. | en |
dc.language | en-us | en |
dc.language.iso | en_US | - |
dc.relation | JOURNAL OF PEDIATRIC SURGERY v.39 n.7 pp.1077-1083 | en |
dc.relation.ispartof | JOURNAL OF PEDIATRIC SURGERY | - |
dc.subject | bcl-2 | en |
dc.subject | p53 | en |
dc.subject | bax | en |
dc.subject | apoptosis | en |
dc.subject | fetal bovine serum | en |
dc.subject | ischemia/ reperfusion | en |
dc.title | Fetal Bovine Serum Suppresses Apoptosis in the Small Intestine after Total Ischemia and Reperfusion in Mice | en |
dc.type | journal article | en |
dc.relation.pages | 1077-1083 | - |
dc.relation.journalvolume | v.39 | - |
dc.relation.journalissue | n.7 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.languageiso639-1 | en_US | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.grantfulltext | none | - |
顯示於: | 醫學系 |
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