Resveratrol Suppresses Angiogenesis in Gliomas: Evaluation by Color Doppler Ultrasound
Resource
ANTICANCER RESEARCH v.26 n.2A pp.1237-1245
Journal
ANTICANCER RESEARCH
Journal Volume
v.26
Journal Issue
n.2A
Pages
1237-1245
Date Issued
2006
Date
2006
Author(s)
CHEN, JIN-CHERNG
CHEN, YUN
LIN, JYH-HERNG
WU, JIANN-MING
TSENG, SHENG-HONG
Abstract
Background: The effects of resveratrol (a natural polyphenol ) on angiogenesis in rat gliomas were investigated by color Doppler ultrasound (CDUS). The correlation among the tumor growth rate, macroscopic angiogenesis measured by CDUS and microscopic angiogenesis was assessed by immunohistochemical staining. Materials and Methods: Fischer rats were subcutaneously inoculated with rat RT-2 glioma cells and treated with resveratrol for 4 weeks. The tumor size was measured and the animal survival followed. CDUS examination was used to measure tumor blood flow shown as the color Doppler vascularity index (CDVI). Immunohistochemical staining of CD31 was carried out to assess the microvessel density (MVD) of the tumors. The CDVI, MVD and tumor size were correlated. Results: Rats treated with resveratrol (40 mg/kg/day) had slower tumor growth rates than those of the control groups (p < 0.05). The CDVI, MVD and tumor size were significantly con-elated (linear regression, p < 0.0001). Conclusion: Resveratrol-suppressed glioma growth was significantly correlated with the inhibition of macroscopic and microscopic angiogenesis.
Subjects
angiogenesis
glioma
resveratrol
color Doppler ultrasound
Type
journal article