|Title:||Tetrandrine Suppresses Tumor Growth and Angiogenesis of Gliomas in Rats||Authors:||CHEN, YUN
|Keywords:||glioma;tetrandrine;antitumor effect;angiogenesis||Issue Date:||2009||Start page/Pages:||2260-2269||Source:||International Journal of Cancer||Abstract:||
Tetrandrine, a bisbenzylisoquinoline alkaloid, has antitumor effects against some cancers, but its effects on gliomas are unknown. In this study, we investigated the effects of tetrandrine on the growth and angiogenesis of rat RT-2 gliomas. We treated RT-2 glioma cells with tetrandrine and then measured cytotoxicity, apoptosis and expression of vascular endothelial growth factor (VEGF). We also examined the cytotoxic effect of tetrandrine on the ECV304 human umbilical vein endothelial cells and the effects of tetrandrine on the in vivo angiogenesis. Tumor size and animal survival were followed in tetrandrine-treated rats with subcutaneous or intracerebral gliomas. Expression of CD 31 in tetrandrine- treated gliomas was followed to study its effect on glioma-induced angiogenesis. Tetrandrine had cytotoxic effects and induced apoptosis of glioma cells in a concentration- and time-dependent manner. Tetrandrine also inhibited the expression of VEGF in glioma cells, induced cytotoxicity effect on the ECV304 cells and suppressed the in vivo angiogenesis. Tetrandrine (150 mg/kg/day) had significant antitumor effects on subcutaneous tumors and led to slower tumor growth rate, longer animal survival time and higher animal survival (p < 0.05). Tetrandrine also affected intracerebral tumors and prolonged animal survival( p < 0.05 ) without affecting survival rate. Immunobistochemical analyses showed that the subcutaneous gliomas from tetrandrine-treated rats had fewer microvessel densities than control rats (p = 0.01). The results demonstrate that tetrandrine is cytotoxic to RT-2 glioma cells, has antitumor effects on subcutaneous and intracerebral gliomas, and inhibits angiogenesis in subcutaneous gliomas. Tetrandrine has potential as a treatment for gliomas.
CD31 antigen; disulfiram; vasculotropin; alanine aminotransferase; antineoplastic agent; aspartate aminotransferase; benzylisoquinoline derivative; creatinine; fibroblast growth factor 2; primer DNA; tetrandrine; angiogenesis; animal experiment; animal model; antineoplastic activity; apoptosis; article; cancer inhibition; cancer survival; cell proliferation; concentration response; controlled study; cytotoxicity; drug effect; endothelium cell; glioma; glioma cell; growth rate; human; human cell; immunohistochemistry; in vivo study; microvasculature; mouse; nonhuman; priority journal; protein expression; rat; survival time; tumor volume; umbilical vein; animal; blood; brain tumor; C57BL mouse; cell division; drug antagonism; Fischer 344 rat; flow cytometry; liver; neovascularization (pathology); nick end labeling; nucleotide sequence; pathology; physiology; small intestine; tumor cell line; urea nitrogen blood level; Alanine Transaminase; Animals; Antineoplastic Agents, Phytogenic; Aspartate Aminotransferases; Base Sequence; Benzylisoquinolines; Blood Urea Nitrogen; Brain Neoplasms; Cell Division; Cell Line, Tumor; Creatinine; DNA Primers; Fibroblast Growth Factor 2; Flow Cytometry; Glioma; Humans; In Situ Nick-End Labeling; Intestine, Small; Liver; Mice; Mice, Inbred C57BL; Neovascularization, Pathologic; Rats; Rats, Inbred F344
|Appears in Collections:||醫學系|
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